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2zrq

From Proteopedia

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[[Image:2zrq.png|left|200px]]
 
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==Crystal structure of S324A-subtilisin==
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The line below this paragraph, containing "STRUCTURE_2zrq", creates the "Structure Box" on the page.
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<StructureSection load='2zrq' size='340' side='right'caption='[[2zrq]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zrq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZRQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_2zrq| PDB=2zrq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zrq OCA], [https://pdbe.org/2zrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zrq RCSB], [https://www.ebi.ac.uk/pdbsum/2zrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zrq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/2zrq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zrq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tk-subtilisin (a subtilisin homologue from Thermococcus kodakaraensis) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide. To analyze the folding mechanism of Tk-subtilisin, the crystal structure of the active site mutant of Tk-subtilisin (S324A-subtilisin*), which was refolded in the presence of Ca2+ and absence of Tk-propeptide, was determined at 2.16A resolution. This structure is essentially the same as that of Tk-subtilisin matured from Pro-Tk-subtilisin. S324A-subtilisin* was refolded with a rate constant of 0.17 and 1.8min(-1) at 30 degrees C in the absence and presence of Tk-propeptide, respectively, indicating that Tk-subtilisin does not require Tk-propeptide for folding but requires it for acceleration of folding.
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===Crystal structure of S324A-subtilisin===
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Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding.,Tanaka S, Takeuchi Y, Matsumura H, Koga Y, Takano K, Kanaya S FEBS Lett. 2008 Nov 26;582(28):3875-8. Epub 2008 Oct 23. PMID:18951896<ref>PMID:18951896</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18951896}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2zrq" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18951896 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18951896}}
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==About this Structure==
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[[2zrq]] is a 1 chain structure of [[Subtilisin]] with sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZRQ OCA].
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==See Also==
==See Also==
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*[[Subtilisin]]
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*[[Subtilisin 3D structures|Subtilisin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:18951896</ref><references group="xtra"/>
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__TOC__
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[[Category: Subtilisin]]
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</StructureSection>
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[[Category: Thermococcus kodakarensis]]
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[[Category: Large Structures]]
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[[Category: Kanaya, S.]]
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[[Category: Thermococcus kodakarensis KOD1]]
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[[Category: Koga, Y.]]
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[[Category: Kanaya S]]
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[[Category: Matsumura, H.]]
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[[Category: Koga Y]]
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[[Category: Takano, K.]]
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[[Category: Matsumura H]]
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[[Category: Takeuchi, Y.]]
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[[Category: Takano K]]
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[[Category: Tanaka, S.]]
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[[Category: Takeuchi Y]]
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[[Category: Calcium]]
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[[Category: Tanaka S]]
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[[Category: Crystal structure]]
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[[Category: Hydrolase]]
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[[Category: Protease]]
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[[Category: Secreted]]
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[[Category: Serine protease]]
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[[Category: Subtilisin]]
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[[Category: Thermococcus kodakaraensis]]
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[[Category: Zymogen]]
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Current revision

Crystal structure of S324A-subtilisin

PDB ID 2zrq

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