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2c7n

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[[Image:2c7n.png|left|200px]]
 
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==Human Rabex-5 residues 1-74 in complex with Ubiquitin==
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The line below this paragraph, containing "STRUCTURE_2c7n", creates the "Structure Box" on the page.
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<StructureSection load='2c7n' size='340' side='right'caption='[[2c7n]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c7n]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C7N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2c7n| PDB=2c7n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7n OCA], [https://pdbe.org/2c7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c7n RCSB], [https://www.ebi.ac.uk/pdbsum/2c7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c7n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RABX5_HUMAN RABX5_HUMAN] Rab effector protein acting as linker between gamma-adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase (By similarity).<ref>PMID:9323142</ref> <ref>PMID:11452015</ref> <ref>PMID:15339665</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/2c7n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c7n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The interaction between ubiquitinated proteins and intracellular proteins harboring ubiquitin binding domains (UBDs) is critical to a multitude of cellular processes. Here, we report that Rabex-5, a guanine nucleotide exchange factor for Rab5, binds to Ub through two independent UBDs. These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated EGFRs. Structural and biochemical characterization of the UBDs of Rabex-5 revealed that one of them (MIU, motif interacting with ubiquitin) binds to Ub with modes superimposable to those of the UIM (ubiquitin-interacting motif):Ub interaction, although in the opposite orientation. The other UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of Ub centered on Asp58(Ub) and distinct from the "canonical" Ile44(Ub)-based surface. The two binding surfaces allow Ub to interact simultaneously with different UBDs, thus opening new perspectives in Ub-mediated signaling.
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===HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN===
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Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin.,Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S, Schneider TR Cell. 2006 Mar 24;124(6):1183-95. Epub 2006 Feb 23. PMID:16499958<ref>PMID:16499958</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16499958}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2c7n" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16499958 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16499958}}
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==About this Structure==
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[[2c7n]] is a 12 chain structure of [[Ubiquitin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7N OCA].
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==See Also==
==See Also==
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*[[Ubiquitin]]
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*[[Rab5 GDP/GTP exchange factor|Rab5 GDP/GTP exchange factor]]
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*[[3D structures of ubiquitin|3D structures of ubiquitin]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:16499958</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Confalioneri, S.]]
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[[Category: Large Structures]]
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[[Category: Fiore, P P.Di.]]
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[[Category: Confalioneri S]]
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[[Category: Magri, L.]]
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[[Category: Di Fiore PP]]
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[[Category: Mapelli, M.]]
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[[Category: Magri L]]
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[[Category: Murachelli, A G.]]
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[[Category: Mapelli M]]
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[[Category: Musacchio, A.]]
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[[Category: Murachelli AG]]
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[[Category: Penengo, L.]]
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[[Category: Musacchio A]]
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[[Category: Polo, S.]]
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[[Category: Penengo L]]
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[[Category: Schneider, T R.]]
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[[Category: Polo S]]
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[[Category: Endocytosis]]
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[[Category: Schneider TR]]
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[[Category: Nuclear protein]]
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[[Category: Polyprotein]]
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[[Category: Protein-binding]]
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[[Category: Ubiquitin binding domain]]
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[[Category: Ubiquitin complex]]
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[[Category: Ubl conjugation]]
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Current revision

Human Rabex-5 residues 1-74 in complex with Ubiquitin

PDB ID 2c7n

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