1t7c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:27, 9 October 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1t7c.png|left|200px]]
 
-
<!--
+
==CRYSTAL STRUCTURE OF THE P1 GLU BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX==
-
The line below this paragraph, containing "STRUCTURE_1t7c", creates the "Structure Box" on the page.
+
<StructureSection load='1t7c' size='340' side='right'caption='[[1t7c]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1t7c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T7C FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_1t7c| PDB=1t7c | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7c OCA], [https://pdbe.org/1t7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t7c RCSB], [https://www.ebi.ac.uk/pdbsum/1t7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t7c ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t7/1t7c_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t7c ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The bovine chymotrypsin-bovine pancreatic trypsin inhibitor (BPTI) interaction belongs to extensively studied models of protein-protein recognition. The accommodation of the inhibitor P1 residue in the S1 binding site of the enzyme forms the hot spot of this interaction. Mutations introduced at the P1 position of BPTI result in a more than five orders of magnitude difference of the association constant values with the protease. To elucidate the structural aspects of the discrimination between different P1 residues, crystal structures of five bovine chymotrypsin-P1 BPTI variant complexes have been determined at pH 7.8 to a resolution below 2 A. The set includes polar (Thr), ionizable (Glu, His), medium-sized aliphatic (Met) and large aromatic (Trp) P1 residues and complements our earlier studies of the interaction of different P1 side-chains with the S1 pocket of chymotrypsin. The structures have been compared to the complexes of proteases with similar and dissimilar P1 preferences, including Streptomyces griseus proteases B and E, human neutrophil elastase, crab collagenase, bovine trypsin and human thrombin. The S1 sites of these enzymes share a common general shape of significant rigidity. Large and branched P1 residues adapt in their complexes similar conformations regardless of the polarity and size differences between their S1 pockets. Conversely, long and flexible residues such as P1 Met are present in the disordered form and display a conformational diversity despite similar inhibitory properties with respect to most enzymes studied. Thus, the S1 specificity profiles of the serine proteases appear to result from the precise complementarity of the P1-S1 interface and minor conformational adjustments occurring upon the inhibitor binding.
-
===CRYSTAL STRUCTURE OF THE P1 GLU BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX===
+
Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants.,Czapinska H, Helland R, Smalas AO, Otlewski J J Mol Biol. 2004 Dec 3;344(4):1005-20. PMID:15544809<ref>PMID:15544809</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_15544809}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1t7c" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 15544809 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_15544809}}
+
-
 
+
-
==About this Structure==
+
-
[[1t7c]] is a 4 chain structure of [[Basic Pancreatic Trypsin Inhibitor]] and [[Chymotrypsin]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7C OCA].
+
==See Also==
==See Also==
-
*[[Basic Pancreatic Trypsin Inhibitor]]
+
*[[BPTI 3D structures|BPTI 3D structures]]
-
*[[Chymotrypsin]]
+
*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:15544809</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Chymotrypsin]]
+
[[Category: Large Structures]]
-
[[Category: Czapinska, H.]]
+
[[Category: Czapinska H]]
-
[[Category: Helland, R.]]
+
[[Category: Helland R]]
-
[[Category: Otlewski, J.]]
+
[[Category: Otlewski J]]
-
[[Category: Smalas, A O.]]
+
[[Category: Smalas AO]]
-
[[Category: Bovine pancreatic trypsin inhibitor]]
+
-
[[Category: Bpti]]
+
-
[[Category: Chymotrypsin]]
+
-
[[Category: Crystal structure]]
+
-
[[Category: Hydrolase/hydrolase inhibitor complex]]
+
-
[[Category: Non-cognate binding]]
+
-
[[Category: Primary specificity]]
+
-
[[Category: Protein-protein interaction]]
+
-
[[Category: S1 pocket]]
+
-
[[Category: Serine proteinase]]
+

Current revision

CRYSTAL STRUCTURE OF THE P1 GLU BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX

PDB ID 1t7c

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t7c"
Personal tools