1kcq

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[[Image:1kcq.png|left|200px]]
 
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==Human Gelsolin Domain 2 with a Cd2+ bound==
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The line below this paragraph, containing "STRUCTURE_1kcq", creates the "Structure Box" on the page.
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<StructureSection load='1kcq' size='340' side='right'caption='[[1kcq]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1kcq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KCQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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{{STRUCTURE_1kcq| PDB=1kcq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kcq OCA], [https://pdbe.org/1kcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kcq RCSB], [https://www.ebi.ac.uk/pdbsum/1kcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kcq ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/GELS_HUMAN GELS_HUMAN] Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:[https://omim.org/entry/105120 105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.<ref>PMID:2157434</ref> <ref>PMID:2153578</ref> <ref>PMID:2176481</ref> <ref>PMID:1338910</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/GELS_HUMAN GELS_HUMAN] Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.<ref>PMID:20393563</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kc/1kcq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kcq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mutations in domain 2 (D2, residues 151-266) of the actin-binding protein gelsolin cause familial amyloidosis-Finnish type (FAF). These mutations, D187N or D187Y, lead to abnormal proteolysis of plasma gelsolin at residues 172-173 and a second hydrolysis at residue 243, resulting in an amyloidogenic fragment. Here we present the structure of human gelsolin D2 at 1.65 A and find that Asp 187 is part of a Cd2+ metal-binding site. Two Ca2+ ions are required for a conformational transition of gelsolin to its active form. Differential scanning calorimetry (DSC) and molecular dynamics (MD) simulations suggest that the Cd2+-binding site in D2 is one of these two Ca2+-binding sites and is essential to the stability of D2. Mutation of Asp 187 to Asn disrupts Ca2+ binding in D2, leading to instabilities upon Ca2+ activation. These instabilities make the domain a target for aberrant proteolysis, thereby enacting the first step in the cascade leading to FAF.
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===Human Gelsolin Domain 2 with a Cd2+ bound===
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Loss of a metal-binding site in gelsolin leads to familial amyloidosis-Finnish type.,Kazmirski SL, Isaacson RL, An C, Buckle A, Johnson CM, Daggett V, Fersht AR Nat Struct Biol. 2002 Feb;9(2):112-6. PMID:11753432<ref>PMID:11753432</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_11753432}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1kcq" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11753432 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11753432}}
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==About this Structure==
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[[1kcq]] is a 1 chain structure of [[Gelsolin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KCQ OCA].
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==See Also==
==See Also==
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*[[Gelsolin]]
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*[[Gelsolin 3D structures|Gelsolin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:11753432</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: An, C.]]
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[[Category: Large Structures]]
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[[Category: Buckle, A.]]
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[[Category: An C]]
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[[Category: Daggett, V.]]
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[[Category: Buckle A]]
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[[Category: Fersht, A R.]]
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[[Category: Daggett V]]
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[[Category: Isaacson, R L.]]
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[[Category: Fersht AR]]
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[[Category: Johnson, C M.]]
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[[Category: Isaacson RL]]
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[[Category: Kazmirski, S L.]]
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[[Category: Johnson CM]]
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[[Category: Actin-binding protein]]
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[[Category: Kazmirski SL]]
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[[Category: Alpha-beta structure]]
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[[Category: Cadmium binding]]
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[[Category: Familial amyloidosis--finnish type]]
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[[Category: Metal binding]]
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[[Category: Structural protein]]
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Current revision

Human Gelsolin Domain 2 with a Cd2+ bound

PDB ID 1kcq

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Proteopedia Page Contributors and Editors (what is this?)

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