3gfv

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[[Image:3gfv.png|left|200px]]
 
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==Crystal Structure of Petrobactin-binding Protein YclQ from Bacillu subtilis==
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The line below this paragraph, containing "STRUCTURE_3gfv", creates the "Structure Box" on the page.
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<StructureSection load='3gfv' size='340' side='right'caption='[[3gfv]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3gfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GFV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3gfv| PDB=3gfv | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gfv OCA], [https://pdbe.org/3gfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gfv RCSB], [https://www.ebi.ac.uk/pdbsum/3gfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gfv ProSAT], [https://www.topsan.org/Proteins/MCSG/3gfv TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YCLQ_BACSU YCLQ_BACSU] Probably part of an ABC transporter complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/3gfv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gfv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Iron deprivation activates the expression of components of the siderophore-mediated iron acquisition systems in Bacillus subtilis, including not only the synthesis and uptake of its siderophore bacillibactin but also expression of multiple ABC transporters for iron scavenging using xenosiderophores. The yclNOPQ operon is shown to encode the complete transporter for petrobactin (PB), a photoreactive 3,4-catecholate siderophore produced by many members of the B. cereus group, including B. anthracis. Isogenic disruption mutants in the yclNOPQ transporter, including permease YclN, ATPase YclP, and a substrate-binding protein YclQ, are unable to use either PB or the photoproduct of FePB (FePB(nu)) for iron delivery and growth, in contrast to the wild-type B. subtilis. Complementation of the mutations with the copies of the respective genes restores this capability. The YclQ receptor binds selectively iron-free and ferric PB, the PB precursor, 3,4-dihydroxybenzoic acid (3,4-DHB), and FePB(nu) with high affinity; the ferric complexes are seen in ESI-MS, implying strong electrostatic interaction between the protein-binding pocket and siderophore. The first structure of a gram-positive siderophore receptor is presented. The 1.75-A crystal structure of YclQ reveals a bilobal periplasmic binding protein (PBP) fold consisting of two alpha/beta/alpha sandwich domains connected by a long alpha-helix with the binding pocket containing conserved positively charged and aromatic residues and large enough to accommodate FePB. Orthologs of the B. subtilis PB-transporter YclNOPQ in PB-producing Bacilli are likely contributors to the pathogenicity of these species and provide a potential target for antibacterial strategies.
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===Crystal Structure of Petrobactin-binding Protein YclQ from Bacillu subtilis===
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Characterization of a Bacillus subtilis transporter for petrobactin, an anthrax stealth siderophore.,Zawadzka AM, Kim Y, Maltseva N, Nichiporuk R, Fan Y, Joachimiak A, Raymond KN Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21854-9. Epub 2009 Dec 2. PMID:19955416<ref>PMID:19955416</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3gfv" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19955416 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19955416}}
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==About this Structure==
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[[3gfv]] is a 2 chain structure of [[ABC transporter]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFV OCA].
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==See Also==
==See Also==
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*[[ABC transporter]]
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:19955416</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
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[[Category: Joachimiak, A.]]
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[[Category: Large Structures]]
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[[Category: Kim, Y.]]
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[[Category: Joachimiak A]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Kim Y]]
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[[Category: Maltseva, N.]]
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[[Category: Maltseva N]]
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[[Category: Raymond, K N.]]
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[[Category: Raymond KN]]
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[[Category: Zawadzka, A M.]]
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[[Category: Zawadzka AM]]
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[[Category: Alpha-beta-sandwich]]
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[[Category: Mcsg]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Secreted]]
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[[Category: Structural genomic]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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[[Category: Unknown function]]
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Current revision

Crystal Structure of Petrobactin-binding Protein YclQ from Bacillu subtilis

PDB ID 3gfv

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