2dog

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[[Image:2dog.gif|left|200px]]<br /><applet load="2dog" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2dog" />
 
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'''Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8'''<br />
 
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==Overview==
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==Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8==
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The RimM protein has been implicated in the maturation of the 30S, ribosomal subunit. It binds to ribosomal protein S19, located in the head, domain of the 30S subunit. Multiple sequence alignments predicted that, RimM possesses two domains in its N- and C-terminal regions. In the, present study, we have produced Thermus thermophilus RimM in both the, full-length form (162 residues) and its N-terminal fragment, spanning, residues 1 to 85, as soluble proteins in Escherichia coli and have, performed structural analyses by nuclear magnetic resonance spectroscopy., Residues 1 to 80 of the RimM protein fold into a single structural domain, adopting a six-stranded beta-barrel fold. On the other hand, the, C-terminal region of RimM (residues 81 to 162) is partly folded in, solution. Analyses of 1H-15N heteronuclear single quantum correlation, spectra revealed that a wide range of residues in the C-terminal region, as well as the residues in the vicinity of a hydrophobic patch in the, N-terminal domain, were dramatically affected upon complex formation with, ribosomal protein S19.
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<StructureSection load='2dog' size='340' side='right'caption='[[2dog]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2dog]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DOG FirstGlance]. <br>
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2DOG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DOG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dog OCA], [https://pdbe.org/2dog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dog RCSB], [https://www.ebi.ac.uk/pdbsum/2dog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dog ProSAT], [https://www.topsan.org/Proteins/RSGI/2dog TOPSAN]</span></td></tr>
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==Reference==
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</table>
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Structural characterization of the ribosome maturation protein, RimM., Suzuki S, Tatsuguchi A, Matsumoto E, Kawazoe M, Kaminishi T, Shirouzu M, Muto Y, Takemoto C, Yokoyama S, J Bacteriol. 2007 Sep;189(17):6397-406. Epub 2007 Jul 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17616598 17616598]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/RIMM_THET8 RIMM_THET8] An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes (By similarity).
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[[Category: Thermus thermophilus]]
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== Evolutionary Conservation ==
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[[Category: Kaminishi, T.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Kawazoe, M.]]
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Check<jmol>
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[[Category: Matsumoto, E.]]
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<jmolCheckbox>
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[[Category: Muto, Y.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/do/2dog_consurf.spt"</scriptWhenChecked>
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Shirouzu, M.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Suzuki, S.]]
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</jmolCheckbox>
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[[Category: Takemoto, C.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dog ConSurf].
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[[Category: Tatsuguchi, A.]]
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<div style="clear:both"></div>
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[[Category: Yokoyama, S.]]
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__TOC__
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[[Category: beta barrel]]
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</StructureSection>
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[[Category: national project on protein structural and functional analyses]]
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[[Category: Large Structures]]
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[[Category: nppsfa]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Kaminishi T]]
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[[Category: rsgi]]
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[[Category: Kawazoe M]]
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[[Category: structural genomics]]
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[[Category: Matsumoto E]]
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[[Category: unknown function]]
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[[Category: Muto Y]]
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[[Category: Shirouzu M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:55:27 2008''
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[[Category: Suzuki S]]
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[[Category: Takemoto C]]
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[[Category: Tatsuguchi A]]
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[[Category: Yokoyama S]]

Current revision

Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8

PDB ID 2dog

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