3fmo

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[[Image:3fmo.png|left|200px]]
 
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==Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19==
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The line below this paragraph, containing "STRUCTURE_3fmo", creates the "Structure Box" on the page.
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<StructureSection load='3fmo' size='340' side='right'caption='[[3fmo]], [[Resolution|resolution]] 2.51&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fmo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FMO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3fmo| PDB=3fmo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fmo OCA], [https://pdbe.org/3fmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fmo RCSB], [https://www.ebi.ac.uk/pdbsum/3fmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fmo ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/NU214_HUMAN NU214_HUMAN] Note=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene. Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
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== Function ==
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[https://www.uniprot.org/uniprot/NU214_HUMAN NU214_HUMAN] May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/3fmo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fmo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Key steps in the export of mRNA from the nucleus to the cytoplasm are the transport through the nuclear pore complex (NPC) and the subsequent remodeling of messenger RNA-protein (mRNP) complexes that occurs at the cytoplasmic side of the NPC. Crucial for these events is the recruitment of the DEAD-box helicase Ddx19 to the cytoplasmic filaments of the NPC that is mediated by the nucleoporin Nup214. Here, we present the crystal structure of the Nup214 N-terminal domain in complex with Ddx19 in its ADP-bound state at 2.5 A resolution. Strikingly, the interaction surfaces are not only evolutionarily conserved but also exhibit strongly opposing surface potentials, with the helicase surface being positively and the Nup214 surface being negatively charged. We speculate that the positively charged surface of the interacting ADP-helicase binds competitively to a segment of mRNA of a linearized mRNP, passing through the NPC on its way to the cytoplasm. As a result, the ADP-helicase would dissociate from Nup214 and replace a single bound protein from the mRNA. One cycle of protein replacement would be accompanied, cooperatively, by nucleotide exchange, ATP hydrolysis, release of the ADP-helicase from mRNA and its rebinding to Nup214. Repeat of these cycles would remove proteins from a mRNP, one at a time, akin to a ratchet mechanism for mRNA export.
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===Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19===
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Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19.,Napetschnig J, Kassube SA, Debler EW, Wong RW, Blobel G, Hoelz A Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3089-94. Epub 2009 Feb 10. PMID:19208808<ref>PMID:19208808</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3fmo" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19208808 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19208808}}
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==About this Structure==
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[[3fmo]] is a 2 chain structure of [[Nucleoporin]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FMO OCA].
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==See Also==
==See Also==
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*[[Nucleoporin]]
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*[[Helicase 3D structures|Helicase 3D structures]]
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*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:19208808</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Blobel, G.]]
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[[Category: Large Structures]]
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[[Category: Debler, E W.]]
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[[Category: Blobel G]]
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[[Category: Hoelz, A.]]
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[[Category: Debler EW]]
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[[Category: Napetschnig, J.]]
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[[Category: Hoelz A]]
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[[Category: Alternative splicing]]
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[[Category: Napetschnig J]]
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[[Category: Atp-binding]]
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[[Category: Beta-propeller]]
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[[Category: Chromosomal rearrangement]]
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[[Category: Cytoplasm]]
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[[Category: Dead box]]
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[[Category: Glycoprotein]]
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[[Category: Helicase]]
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[[Category: Hydrolase]]
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[[Category: Membrane]]
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[[Category: Mrna export]]
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[[Category: Mrna transport]]
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[[Category: Nuclear pore complex]]
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[[Category: Nuclear porin]]
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[[Category: Nucleocytoplasmic transport]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleus]]
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[[Category: Oncoprotein/hydrolase complex]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Protein interaction]]
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[[Category: Protein transport]]
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[[Category: Protein transport/hydrolase complex]]
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[[Category: Proto-oncogene]]
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[[Category: Rna-binding]]
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[[Category: Translocation]]
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[[Category: Transport]]
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Current revision

Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19

PDB ID 3fmo

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