2k5x

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[[Image:2k5x.png|left|200px]]
 
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==Chemical shift structure of COLICIN E9 DNASE domain with its cognate immunity protein IM9==
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The line below this paragraph, containing "STRUCTURE_2k5x", creates the "Structure Box" on the page.
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<StructureSection load='2k5x' size='340' side='right'caption='[[2k5x]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2k5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K5X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k5x OCA], [https://pdbe.org/2k5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k5x RCSB], [https://www.ebi.ac.uk/pdbsum/2k5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k5x ProSAT]</span></td></tr>
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{{STRUCTURE_2k5x| PDB=2k5x | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMM9_ECOLX IMM9_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE9 encoding colicin E9, against colicin E9, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k5/2k5x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k5x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nuclear magnetic resonance (NMR) spectroscopy provides a range of powerful techniques for determining the structures and the dynamics of proteins. The high-resolution determination of the structures of protein-protein complexes, however, is still a challenging problem for this approach, since it can normally provide only a limited amount of structural information at protein-protein interfaces. We present here the determination using NMR chemical shifts of the structure (PDB code 2K5X ) of the cytotoxic endonuclease domain from bacterial toxin colicin (E9) in complex with its cognate immunity protein (Im9). In order to achieve this result, we introduce the CamDock method, which combines a flexible docking procedure with a refinement that exploits the structural information provided by chemical shifts. The results that we report thus indicate that chemical shifts can be used as structural restraints for the determination of the conformations of protein complexes that are difficult to obtain by more standard NMR approaches.
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===Chemical shift structure of COLICIN E9 DNASE domain with its cognate immunity protein IM9===
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Structure Determination of Protein-Protein Complexes Using NMR Chemical Shifts: Case of an Endonuclease Colicin-Immunity Protein Complex.,Montalvao RW, Cavalli A, Salvatella X, Blundell TL, Vendruscolo M J Am Chem Soc. 2008 Nov 4. PMID:18980319<ref>PMID:18980319</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_18980319}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2k5x" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18980319 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18980319}}
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==About this Structure==
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[[2k5x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K5X OCA].
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==See Also==
==See Also==
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*[[Colicin]]
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*[[Colicin 3D structures|Colicin 3D structures]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:18980319</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Cavalli, A.]]
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[[Category: Large Structures]]
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[[Category: Montalvao, R W.]]
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[[Category: Cavalli A]]
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[[Category: Vendruscolo, M.]]
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[[Category: Montalvao RW]]
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[[Category: Antibiotic]]
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[[Category: Vendruscolo M]]
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[[Category: Antimicrobial]]
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[[Category: Bacteriocin]]
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[[Category: Bacteriocin immunity]]
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[[Category: Colicin e9]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase]]
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[[Category: Immune system/hydrolase complex]]
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[[Category: Immunity protein im9]]
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[[Category: Metal-binding]]
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[[Category: Nuclease]]
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[[Category: Plasmid]]
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[[Category: Zinc]]
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Current revision

Chemical shift structure of COLICIN E9 DNASE domain with its cognate immunity protein IM9

PDB ID 2k5x

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