3nc0

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[[Image:3nc0.png|left|200px]]
 
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==Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal II)==
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The line below this paragraph, containing "STRUCTURE_3nc0", creates the "Structure Box" on the page.
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<StructureSection load='3nc0' size='340' side='right'caption='[[3nc0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nc0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NC0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=IPH:PHENOL'>IPH</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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{{STRUCTURE_3nc0| PDB=3nc0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nc0 OCA], [https://pdbe.org/3nc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nc0 RCSB], [https://www.ebi.ac.uk/pdbsum/3nc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nc0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XPO1_MOUSE XPO1_MOUSE] Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity).<ref>PMID:20921223</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Classic nuclear export signals (NESs) confer CRM1-dependent nuclear export. Here we present crystal structures of the RanGTP-CRM1 complex alone and bound to the prototypic PKI or HIV-1 Rev NESs. These NESs differ markedly in the spacing of their key hydrophobic (Phi) residues, yet CRM1 recognizes them with the same rigid set of five Phi pockets. The different Phi spacings are compensated for by different conformations of the bound NESs: in the case of PKI, an alpha-helical conformation, and in the case of Rev, an extended conformation with a critical proline docking into a Phi pocket. NMR analyses of CRM1-bound and CRM1-free PKI NES suggest that CRM1 selects NES conformers that pre-exist in solution. Our data lead to a new structure-based NES consensus, and explain why NESs differ in their affinities for CRM1 and why supraphysiological NESs bind the exportin so tightly.
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===Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal II)===
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NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1.,Guttler T, Madl T, Neumann P, Deichsel D, Corsini L, Monecke T, Ficner R, Sattler M, Gorlich D Nat Struct Mol Biol. 2010 Oct 24. PMID:20972448<ref>PMID:20972448</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nc0" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20972448}}, adds the Publication Abstract to the page
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*[[Exportin 3D structures|Exportin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20972448 is the PubMed ID number.
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20972448}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[3nc0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NC0 OCA].
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==Reference==
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<ref group="xtra">PMID:20972448</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Corsini, L.]]
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[[Category: Corsini L]]
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[[Category: Deichsel, D.]]
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[[Category: Deichsel D]]
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[[Category: Ficner, R.]]
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[[Category: Ficner R]]
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[[Category: Gorlich, D.]]
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[[Category: Gorlich D]]
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[[Category: Guttler, T.]]
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[[Category: Guttler T]]
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[[Category: Madl, T.]]
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[[Category: Madl T]]
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[[Category: Monecke, T.]]
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[[Category: Monecke T]]
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[[Category: Neumann, P.]]
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[[Category: Neumann P]]
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[[Category: Sattler, M.]]
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[[Category: Sattler M]]
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[[Category: Gtp-binding protein-transport protein complex]]
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[[Category: Protein transport]]
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Current revision

Crystal structure of the HIV-1 Rev NES-CRM1-RanGTP nuclear export complex (crystal II)

PDB ID 3nc0

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