2x6t

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[[Image:2x6t.png|left|200px]]
 
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==AGME bound to ADP-B-mannose==
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The line below this paragraph, containing "STRUCTURE_2x6t", creates the "Structure Box" on the page.
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<StructureSection load='2x6t' size='340' side='right'caption='[[2x6t]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x6t]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X6T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.36&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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{{STRUCTURE_2x6t| PDB=2x6t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x6t OCA], [https://pdbe.org/2x6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x6t RCSB], [https://www.ebi.ac.uk/pdbsum/2x6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x6t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HLDD_ECOLI HLDD_ECOLI] Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose.<ref>PMID:6337148</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x6/2x6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x6t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteria synthesise a wide array of unusual carbohydrate molecules which they use in a variety of ways. The carbohydrate L-glycero-D-manno-heptose is an important component of lipopolysaccharide and is synthesised in a complex series of enzymatic steps. One step involves the epimerisation at the C6'' position converting ADP-D-glycero-D-manno-heptose into ADP-L-glycero-D-manno-heptose. The enzyme responsible is a member of the short chain dehydrogenase superfamily, known as ADP-L-glycero-D-manno-heptose 6-epimerase (AGME). The structure of the enzyme was known but the arrangement of the catalytic site with respect to the substrate is unclear. We now report the structure of AGME bound to a substrate mimic, ADP-beta-D-mannose, which has the same stereochemical configuration as the substrate. The complex identifies the key residues and allows mechanistic insight into this novel enzyme.
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===AGME BOUND TO ADP-B-MANNOSE===
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The crystal structure of the Y140F mutant of ADP-L-glycero-D-manno-heptose 6-epimerase bound to ADP-beta-D-mannose suggests a one base mechanism.,Kowatz T, Morrison JP, Tanner ME, Naismith JH Protein Sci. 2010 May 5. PMID:20506248<ref>PMID:20506248</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20506248}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x6t" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20506248 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20506248}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2x6t]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X6T OCA].
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==Reference==
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<ref group="xtra">PMID:20506248</ref><references group="xtra"/>
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[[Category: ADP-glyceromanno-heptose 6-epimerase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Kowatz, T.]]
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[[Category: Large Structures]]
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[[Category: Morrison, J P.]]
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[[Category: Kowatz T]]
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[[Category: Naismith, J H.]]
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[[Category: Morrison JP]]
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[[Category: Tanner, M E.]]
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[[Category: Naismith JH]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Tanner ME]]
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[[Category: Isomerase]]
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[[Category: Stress response]]
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Current revision

AGME bound to ADP-B-mannose

PDB ID 2x6t

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