2x58

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[[Image:2x58.png|left|200px]]
 
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==The crystal structure of MFE1 liganded with CoA==
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The line below this paragraph, containing "STRUCTURE_2x58", creates the "Structure Box" on the page.
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<StructureSection load='2x58' size='340' side='right'caption='[[2x58]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X58 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2x58| PDB=2x58 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x58 OCA], [https://pdbe.org/2x58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x58 RCSB], [https://www.ebi.ac.uk/pdbsum/2x58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x58 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ECHP_RAT ECHP_RAT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x5/2x58_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x58 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the full-length rat peroxisomal multifunctional enzyme, type 1 (rpMFE1), has been determined at 2.8 A resolution. This enzyme has three catalytic activities and two active sites. The N-terminal part has the crotonase fold, which builds the active site for the Delta(3),Delta(2)-enoyl-CoA isomerase and the Delta(2)-enoyl-CoA hydratase-1 catalytic activities, and the C-terminal part has the (3S)-hydroxyacyl-CoA dehydrogenase fold and makes the (3S)-hydroxyacyl-CoA dehydrogenase active site. rpMFE1 is a multidomain protein having five domains (A-E). The crystal structure of full-length rpMFE1 shows a flexible arrangement of the A-domain with respect to the B-E-domains. Because of a hinge region near the end of the A-domain, two different positions of the A-domain were observed for the two protein molecules (A and B) of the asymmetric unit. In the most closed conformation, the mode of binding of CoA is stabilized by domains A and B (helix-10), as seen in other crotonase fold members. Domain B, although functionally belonging to the N-terminal part, is found tightly associated with the C-terminal part, i.e. fixed to the E-domain. The two active sites of rpMFE1 are approximately 40 A apart, separated by a tunnel, characterized by an excess of positively charged side chains. Comparison of the structures of rpMFE1 with the monofunctional crotonase and (3S)-hydroxyacyl-CoA dehydrogenase superfamily enzymes, as well as with the bacterial alpha(2)beta(2)-fatty acid oxidation multienzyme complex, reveals that this tunnel could be important for substrate channeling, as observed earlier on the basis of the kinetics of rpMFE1 purified from rat liver.
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===THE CRYSTAL STRUCTURE OF MFE1 LIGANDED WITH COA===
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Crystal structure of liganded rat peroxisomal multifunctional enzyme type 1: a flexible molecule with two interconnected active sites.,Kasaragod P, Venkatesan R, Kiema TR, Hiltunen JK, Wierenga RK J Biol Chem. 2010 Jul 30;285(31):24089-98. Epub 2010 May 12. PMID:20463028<ref>PMID:20463028</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2x58" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20463028}}, adds the Publication Abstract to the page
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*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20463028 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20463028}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2x58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X58 OCA].
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==Reference==
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<ref group="xtra">PMID:20463028</ref><references group="xtra"/>
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Hiltunen, J K.]]
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[[Category: Hiltunen JK]]
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[[Category: Kasaragod, P.]]
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[[Category: Kasaragod P]]
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[[Category: Kiema, T R.]]
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[[Category: Kiema TR]]
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[[Category: Venkatesan, R.]]
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[[Category: Venkatesan R]]
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[[Category: Wierenga, R K.]]
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[[Category: Wierenga RK]]
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[[Category: Beta oxidation pathway]]
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[[Category: Fatty acid metabolism]]
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[[Category: Isomerase]]
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[[Category: Lipid metabolism]]
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[[Category: Lyase]]
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[[Category: Oxidoreductase]]
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[[Category: Peroxisome]]
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Current revision

The crystal structure of MFE1 liganded with CoA

PDB ID 2x58

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