1j5m

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[[Image:1j5m.png|left|200px]]
 
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==SOLUTION STRUCTURE OF THE SYNTHETIC 113CD_3 BETA_N DOMAIN OF LOBSTER METALLOTHIONEIN-1==
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The line below this paragraph, containing "STRUCTURE_1j5m", creates the "Structure Box" on the page.
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<StructureSection load='1j5m' size='340' side='right'caption='[[1j5m]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1j5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homarus_americanus Homarus americanus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hzr 1hzr]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J5M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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{{STRUCTURE_1j5m| PDB=1j5m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j5m OCA], [https://pdbe.org/1j5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j5m RCSB], [https://www.ebi.ac.uk/pdbsum/1j5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j5m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MT1_HOMAM MT1_HOMAM] Metallothioneins have a high content of cysteine residues that bind various heavy metals. The different forms of lobster metallothioneins may have different biological functions. Class I MTS in marine crustacea are involved in the sequestration of elevated levels of heavy-metal ions. Binds 6 metal ions. Known to bind cadmium.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structures of the isolated Cd(3)beta domains from Homarus americanus metallothionein have been determined by NMR methods in order to establish a set of beta-domain structures for comparative analysis. First, it was determined that the Cd-cysteine connectivities forming the Cd(3)S(9) metal center were identical to those observed for the beta(N) domain in the native holoprotein. Time- and temperature-dependence studies of the (113)Cd and (1)H 1D-NMR spectra indicated that the beta(N) domain undergoes slow conformational changes before reaching an equilibrium structure. In addition to structural information provided by the metal-to-cysteine connectivities, Phi, chi(1) and chi(2) angle constraints, three H(N...)S hydrogen bond interactions were also determined from a long-range optimized (1)H(N)-(113)Cd HMQC experiment. A simulated annealing protocol was applied to the distance and angle constraints obtained from the 2D-NMR experiments to calculate the three-dimensional structure of the synthetic Cd(3)beta(N) domain of lobster metallothionein. Structure-reactivity relationships are proposed for the reactions of Cd(3)beta domains with 5,5'-dithiobis(2-nitrobenzoate), based on comparisons of surface exposure of sulfur atoms of the lobster and rabbit Cd(3)beta domain structures. Finally, the surface exposure of the beta domains of lobster is compared with beta domains from mammalian metallothioneins.
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===SOLUTION STRUCTURE OF THE SYNTHETIC 113CD_3 BETA_N DOMAIN OF LOBSTER METALLOTHIONEIN-1===
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Structure of the (113)Cd(3)beta domains from Homarus americanus metallothionein-1: hydrogen bonding and solvent accessibility of sulfur atoms.,Munoz A, Forsterling FH, Shaw CF 3rd, Petering DH J Biol Inorg Chem. 2002 Sep;7(7-8):713-24. Epub 2002 Mar 13. PMID:12203008<ref>PMID:12203008</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1j5m" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12203008 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12203008}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Homarus americanus]]
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[[1j5m]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hzr 1hzr]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5M OCA].
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[[Category: Large Structures]]
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[[Category: Forsterling FH]]
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==Reference==
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[[Category: Munoz A]]
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<ref group="xtra">PMID:12203008</ref><references group="xtra"/>
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[[Category: Petering DH]]
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[[Category: Forsterling, F H.]]
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[[Category: Shaw III CF]]
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[[Category: III, C F.Shaw.]]
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[[Category: Munoz, A.]]
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[[Category: Petering, D H.]]
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[[Category: 113cd-nmr]]
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[[Category: 2d-nmr]]
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[[Category: Beta-domain]]
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[[Category: Conformational change]]
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[[Category: Hn-s-hydrogen bond]]
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[[Category: Metal binding protein]]
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[[Category: Metal-sulfur-cluster]]
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[[Category: Metallothionein]]
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Current revision

SOLUTION STRUCTURE OF THE SYNTHETIC 113CD_3 BETA_N DOMAIN OF LOBSTER METALLOTHIONEIN-1

PDB ID 1j5m

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