2d5i

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[[Image:2d5i.gif|left|200px]]<br /><applet load="2d5i" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2d5i, resolution 2.20&Aring;" />
 
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'''The crystal structure of AzoR (Azo Reductase) from Escherichia coli'''<br />
 
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==Overview==
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==The crystal structure of AzoR (Azo Reductase) from Escherichia coli==
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The crystal structure of AzoR (azoreductase) has been determined in, complex with FMN for two different crystal forms at 1.8 and 2.2 A, resolution. AzoR is an oxidoreductase isolated from Escherichia coli as a, protein responsible for the degradation of azo compounds. This enzyme is, an FMN-dependent NADH-azoreductase and catalyzes the reductive cleavage of, azo groups by a ping-pong mechanism. The structure suggests that AzoR acts, in a homodimeric state forming the two identical catalytic sites to which, both monomers contribute. The structure revealed that each monomer of AzoR, has a flavodoxin-like structure, without the explicit overall amino acid, sequence homology. Superposition of the structures from the two different, crystal forms revealed the conformational change and suggested a mechanism, for accommodating substrates of different size. Furthermore, comparison of, the active site structure with that of NQO1 complexed with substrates, provides clues to the possible substrate-binding mechanism of AzoR.
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<StructureSection load='2d5i' size='340' side='right'caption='[[2d5i]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2d5i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D5I FirstGlance]. <br>
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2D5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Azobenzene_reductase Azobenzene reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.1.6 1.7.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D5I OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d5i OCA], [https://pdbe.org/2d5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d5i RCSB], [https://www.ebi.ac.uk/pdbsum/2d5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d5i ProSAT]</span></td></tr>
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Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms., Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M, J Biol Chem. 2006 Jul 21;281(29):20567-76. Epub 2006 May 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16684776 16684776]
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</table>
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[[Category: Azobenzene reductase]]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/AZOR_ECOLI AZOR_ECOLI] Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity. The enzyme can reduce ethyl red and methyl red, but is not able to convert sulfonated azo dyes.<ref>PMID:2168383</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Ito, K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Tanokura, M.]]
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Check<jmol>
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[[Category: FMN]]
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<jmolCheckbox>
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[[Category: GOL]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d5/2d5i_consurf.spt"</scriptWhenChecked>
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[[Category: azo reductase]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: oxidoreductase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:06:17 2008''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d5i ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Ito K]]
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[[Category: Tanokura M]]

Current revision

The crystal structure of AzoR (Azo Reductase) from Escherichia coli

PDB ID 2d5i

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