2qf4

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(New page: 200px<br /><applet load="2qf4" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qf4, resolution 1.200&Aring;" /> '''High resolution str...)
Current revision (09:15, 21 February 2024) (edit) (undo)
 
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[[Image:2qf4.jpg|left|200px]]<br /><applet load="2qf4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qf4, resolution 1.200&Aring;" />
 
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'''High resolution structure of the major periplasmic domain from the cell shape-determining filament MreC (orthorhombic form)'''<br />
 
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==Overview==
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==High resolution structure of the major periplasmic domain from the cell shape-determining filament MreC (orthorhombic form)==
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Bacterial cell shape is dictated by the cell wall, a plastic structure, that must adapt to growth and division whilst retaining its function as a, selectively permeable barrier. The modulation of cell wall structure is, achieved by a variety of enzymatic functions, all of which must be, spatially regulated in a precise manner. The membrane-spanning essential, protein MreC has been identified as the central hub in this process, linking the bacterial cytoskeleton to a variety of cell wall-modifying, enzymes. Additionally, MreC can form filaments, believed to run, perpendicularly to the membrane. We present here the 1.2 A resolution, crystal structure of the major periplasmic domain of Streptococcus, pneumoniae MreC. The protein shows a novel arrangement of two, barrel-shaped domains, one of which shows homology to a known protein, oligomerization motif, with the other resembling a catalytic domain from a, bacterial protease. We discuss the implications of these results for MreC, function, and detail the structural features of the molecule that may be, responsible for the binding of partner proteins.
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<StructureSection load='2qf4' size='340' side='right'caption='[[2qf4]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2qf4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QF4 FirstGlance]. <br>
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2QF4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae_r6 Streptococcus pneumoniae r6] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QF4 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qf4 OCA], [https://pdbe.org/2qf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qf4 RCSB], [https://www.ebi.ac.uk/pdbsum/2qf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qf4 ProSAT]</span></td></tr>
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High-resolution Structure of the Major Periplasmic Domain from the Cell Shape-determining Filament MreC., Lovering AL, Strynadka NC, J Mol Biol. 2007 Sep 28;372(4):1034-44. Epub 2007 Jul 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17707860 17707860]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Streptococcus pneumoniae r6]]
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[https://www.uniprot.org/uniprot/MREC_STRR6 MREC_STRR6] Involved in formation and maintenance of cell shape.[UniProtKB:Q01466]
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[[Category: Lovering, A.L.]]
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== Evolutionary Conservation ==
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[[Category: Strynadka, N.C.J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: EDO]]
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Check<jmol>
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[[Category: SO4]]
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<jmolCheckbox>
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[[Category: filament a-lytic protease fold]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qf/2qf4_consurf.spt"</scriptWhenChecked>
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[[Category: structural protein]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:08:19 2008''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qf4 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptococcus pneumoniae R6]]
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[[Category: Lovering AL]]
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[[Category: Strynadka NCJ]]

Current revision

High resolution structure of the major periplasmic domain from the cell shape-determining filament MreC (orthorhombic form)

PDB ID 2qf4

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