3ncx

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[[Image:3ncx.jpg|left|200px]]
 
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==Crystal structure of EHEC O157:H7 intimin mutant==
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The line below this paragraph, containing "STRUCTURE_3ncx", creates the "Structure Box" on the page.
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<StructureSection load='3ncx' size='340' side='right'caption='[[3ncx]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ncx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._TW14359 Escherichia coli O157:H7 str. TW14359]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NCX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ncx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ncx OCA], [https://pdbe.org/3ncx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ncx RCSB], [https://www.ebi.ac.uk/pdbsum/3ncx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ncx ProSAT]</span></td></tr>
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{{STRUCTURE_3ncx| PDB=3ncx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57] Necessary for the production of attaching and effacing lesions on tissue culture cells.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Enterohaemorrhagic E. coli (EHEC) O157:H7 is a primary food-borne bacterial pathogen capable of causing life-threatening human infections which poses a serious challenge to public health worldwide. Intimin, the bacterial outer-membrane protein, plays a key role in the initiating process of EHEC infection. This activity is dependent upon translocation of the intimin receptor (Tir), the intimin binding partner of the bacteria-encoded host cell surface protein. Intimin has attracted considerable attention due to its potential function as an antibacterial drug target. Here, we report the crystal structure of the Tir-binding domain of intimin (Int188) from E. coli O157:H7 at 2.8 A resolution, together with a mutant (IntN916Y) at 2.6 A. We also built the structural model of EHEC intimin-Tir complex and analyzed the key binding residues. It suggested that the binding pattern of intimin and Tir between EHEC and Enteropathogenic E. coli (EPEC) adopt a similar mode and they can complement with each other. Detailed structural comparison indicates that there are four major points of structural variations between EHEC and EPEC intimins: one in Domain I (Ig-like domain), the other three located in Domain II (C-type lectin-like domain). These variations result in different binding affinities. These findings provide structural insight into the binding pattern of intimin to Tir and the molecular mechanism of EHEC O157: H7.
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===Crystal structure of EHEC O157:H7 intimin mutant===
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Crystal structure of EHEC intimin: insights into the complementarity between EPEC and EHEC.,Yi Y, Ma Y, Gao F, Mao X, Peng H, Feng Y, Fan Z, Wang G, Guo G, Yan J, Zeng H, Zou Q, Gao GF PLoS One. 2010 Dec 16;5(12):e15285. PMID:21179574<ref>PMID:21179574</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3ncx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NCX OCA].
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<div class="pdbe-citations 3ncx" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli o157:h7]]
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== References ==
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[[Category: Gao, F.]]
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<references/>
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[[Category: Gao, G F.]]
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__TOC__
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[[Category: Yi, Y.]]
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</StructureSection>
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[[Category: Zou, Q M.]]
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[[Category: Escherichia coli O157:H7 str. TW14359]]
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[[Category: Large Structures]]
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[[Category: Gao F]]
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[[Category: Gao GF]]
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[[Category: Yi Y]]
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[[Category: Zou QM]]

Current revision

Crystal structure of EHEC O157:H7 intimin mutant

PDB ID 3ncx

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