3mhz

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[[Image:3mhz.png|left|200px]]
 
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==1.7A structure of 2-fluorohistidine labeled Protective Antigen==
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The line below this paragraph, containing "STRUCTURE_3mhz", creates the "Structure Box" on the page.
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<StructureSection load='3mhz' size='340' side='right'caption='[[3mhz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3mhz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MHZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HF:2-FLUORO-L-HISTIDINE'>2HF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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{{STRUCTURE_3mhz| PDB=3mhz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mhz OCA], [https://pdbe.org/3mhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mhz RCSB], [https://www.ebi.ac.uk/pdbsum/3mhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mhz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/3mhz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mhz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The protective antigen (PA) component of the anthrax toxin forms pores within the low pH environment of host endosomes through mechanisms that are poorly understood. It has been proposed that pore formation is dependent on histidine protonation. In previous work, we biosynthetically incorporated 2-fluorohistidine (2-FHis), an isosteric analogue of histidine with a significantly reduced pK(a) ( approximately 1), into PA and showed that the pH-dependent conversion from the soluble prepore to a pore was unchanged. However, we also observed that 2-FHisPA was nonfunctional in the ability to mediate cytotoxicity of CHO-K1 cells by LF(N)-DTA and was defective in translocation through planar lipid bilayers. Here, we show that the defect in cytotoxicity is due to both a defect in translocation and, when bound to the host cellular receptor, an inability to undergo low pH-induced pore formation. Combining X-ray crystallography with hydrogen-deuterium (H-D) exchange mass spectrometry, our studies lead to a model in which hydrogen bonds to the histidine ring are strengthened by receptor binding. The combination of both fluorination and receptor binding is sufficient to block low pH-induced pore formation.
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===1.7A structure of 2-fluorohistidine labeled Protective Antigen===
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Evidence that histidine protonation of receptor-bound anthrax protective antigen is a trigger for pore formation.,Wimalasena DS, Janowiak BE, Lovell S, Miyagi M, Sun J, Zhou H, Hajduch J, Pooput C, Kirk KL, Battaile KP, Bann JG Biochemistry. 2010 Aug 24;49(33):6973-83. PMID:20672855<ref>PMID:20672855</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20672855}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3mhz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20672855 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20672855}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3mhz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MHZ OCA].
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==Reference==
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<ref group="xtra">PMID:20672855</ref><references group="xtra"/>
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[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
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[[Category: Bann, J G.]]
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[[Category: Large Structures]]
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[[Category: Battaile, K P.]]
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[[Category: Bann JG]]
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[[Category: Hajduch, J.]]
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[[Category: Battaile KP]]
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[[Category: Janowiak, B E.]]
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[[Category: Hajduch J]]
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[[Category: Kirk, K L.]]
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[[Category: Janowiak BE]]
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[[Category: Lovell, S.]]
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[[Category: Kirk KL]]
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[[Category: Miyagi, M.]]
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[[Category: Lovell S]]
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[[Category: Pooput, C.]]
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[[Category: Miyagi M]]
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[[Category: Sun, J.]]
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[[Category: Pooput C]]
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[[Category: Wimalasena, D S.]]
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[[Category: Sun J]]
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[[Category: Wimalasena DS]]

Current revision

1.7A structure of 2-fluorohistidine labeled Protective Antigen

PDB ID 3mhz

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