3piq

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[[Image:3piq.png|left|200px]]
 
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==Crystal structure of human 2909 Fab, a quaternary structure-specific antibody against HIV-1==
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The line below this paragraph, containing "STRUCTURE_3piq", creates the "Structure Box" on the page.
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<StructureSection load='3piq' size='340' side='right'caption='[[3piq]], [[Resolution|resolution]] 3.33&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3piq]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PIQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.325&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3piq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3piq OCA], [https://pdbe.org/3piq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3piq RCSB], [https://www.ebi.ac.uk/pdbsum/3piq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3piq ProSAT]</span></td></tr>
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{{STRUCTURE_3piq| PDB=3piq | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Monoclonal antibody 2909 belongs to a class of potently neutralizing antibodies that recognizes quaternary epitopes on HIV-1. Some members of this class, such as 2909, are strain-specific, while others, such as antibody PG16, are broadly neutralizing; all, however, recognize a region on the gp120 envelope glycoprotein that includes two loops (V2 and V3) and forms appropriately only in the oligomeric HIV-1 spike (gp1203/gp413). Here we present the crystal structure of 2909 and report structure-function analysis with antibody chimeras composed of 2909 and other members of this antibody class. The 2909 structure was dominated by a heavy chain 3(rd)-complementarity-determining region (CDR H3) of 21-residues, which comprised 36% of the combining surface and formed a beta-hairpin club extending approximately 20A-beyond the rest of the antibody. Sequence analysis and mass spectrometry identified sites of tyrosine sulfation, at the middle and top of the CDR H3; substitution with phenylalanine either ablated (middle substitution) or substantially diminished (top substitution) neutralization. Chimeric antibodies composed of heavy and light chains, exchanged between 2909 and other members of the class, indicated a substantial lack of complementation. Comparison of 2909 to PG16 (which is tyrosine sulfated and the only other member of the class for which a structure has previously been reported) showed that both utilize protruding, anionic CDR H3s for recognition. Thus, despite some diversity, members of this class share structural and functional similarities, with conserved features of CDR H3 subdomain likely reflecting conserved solutions by the human immune system for recognition of a quaternary site of HIV-1 vulnerability.
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===Crystal structure of human 2909 Fab, a quaternary structure-specific antibody against HIV-1===
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Crystal structure of human antibody 2909 reveals conserved features of quaternary-specific antibodies that potently neutralize HIV-1.,Changela A, Wu X, Yang Y, Zhang B, Zhu J, Nardone GA, O'Dell S, Pancera M, Gorny MK, Phogat S, Robinson JE, Stamatatos L, Zolla-Pazner S, Mascola JR, Kwong PD J Virol. 2010 Dec 29. PMID:21191009<ref>PMID:21191009</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3piq" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21191009 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21191009}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3piq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PIQ OCA].
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==Reference==
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<ref group="xtra">PMID:21191009</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Changela, A.]]
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[[Category: Large Structures]]
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[[Category: Gorny, M K.]]
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[[Category: Changela A]]
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[[Category: Kwong, P D.]]
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[[Category: Gorny MK]]
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[[Category: Zolla-Pazner, S.]]
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[[Category: Kwong PD]]
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[[Category: Zolla-Pazner S]]

Current revision

Crystal structure of human 2909 Fab, a quaternary structure-specific antibody against HIV-1

PDB ID 3piq

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