2e7p

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(New page: 200px<br /><applet load="2e7p" size="350" color="white" frame="true" align="right" spinBox="true" caption="2e7p, resolution 2.10&Aring;" /> '''Crystal structure of...)
Current revision (13:49, 13 March 2024) (edit) (undo)
 
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[[Image:2e7p.jpg|left|200px]]<br /><applet load="2e7p" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2e7p, resolution 2.10&Aring;" />
 
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'''Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides'''<br />
 
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==Overview==
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==Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides==
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When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC, active site) exists as a dimeric iron-sulfur-containing holoprotein or as, a monomeric apoprotein in solution. Analytical and spectroscopic studies, of wild-type protein and site-directed variants and structural, characterization of the holoprotein by using x-ray crystallography, indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster, that is ligated by the catalytic cysteines of two glutaredoxins and the, cysteines of two glutathiones. Mutagenesis data on a variety of poplar, glutaredoxins suggest that the incorporation of an iron-sulfur cluster, could be a general feature of plant glutaredoxins possessing a glycine, adjacent to the catalytic cysteine. In light of these results, the, possible involvement of plant glutaredoxins in oxidative stress sensing or, iron-sulfur biosynthesis is discussed with respect to their intracellular, localization.
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<StructureSection load='2e7p' size='340' side='right'caption='[[2e7p]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2e7p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula_x_Populus_tremuloides Populus tremula x Populus tremuloides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E7P FirstGlance]. <br>
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2E7P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Populus_tremula_x_populus_tremuloides Populus tremula x populus tremuloides] with <scene name='pdbligand=FES:'>FES</scene> and <scene name='pdbligand=GTT:'>GTT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7P OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e7p OCA], [https://pdbe.org/2e7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e7p RCSB], [https://www.ebi.ac.uk/pdbsum/2e7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e7p ProSAT]</span></td></tr>
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Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1., Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP, Proc Natl Acad Sci U S A. 2007 May 1;104(18):7379-84. Epub 2007 Apr 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17460036 17460036]
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</table>
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[[Category: Populus tremula x populus tremuloides]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q5PSJ1_POPPZ Q5PSJ1_POPPZ]
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[[Category: Aimoto, S.]]
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== Evolutionary Conservation ==
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[[Category: Hase, T.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Jacquot, J.P.]]
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Check<jmol>
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[[Category: Kawakami, T.]]
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<jmolCheckbox>
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[[Category: Kusunoki, M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/2e7p_consurf.spt"</scriptWhenChecked>
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[[Category: Rouhier, N.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Takahashi, T.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Unno, H.]]
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</jmolCheckbox>
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[[Category: FES]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e7p ConSurf].
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[[Category: GTT]]
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<div style="clear:both"></div>
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[[Category: electron transport]]
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__TOC__
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[[Category: glutaredoxin]]
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</StructureSection>
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[[Category: poplar]]
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[[Category: Large Structures]]
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[[Category: thioredoxin fold]]
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[[Category: Populus tremula x Populus tremuloides]]
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[[Category: Aimoto S]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:25:04 2008''
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[[Category: Hase T]]
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[[Category: Jacquot JP]]
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[[Category: Kawakami T]]
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[[Category: Kusunoki M]]
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[[Category: Rouhier N]]
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[[Category: Takahashi T]]
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[[Category: Unno H]]

Current revision

Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides

PDB ID 2e7p

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