2r0g

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(New page: 200px<br /><applet load="2r0g" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r0g, resolution 2.37&Aring;" /> '''Chromopyrrolic acid-...)
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[[Image:2r0g.jpg|left|200px]]<br /><applet load="2r0g" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2r0g, resolution 2.37&Aring;" />
 
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'''Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c'''<br />
 
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==Overview==
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==Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c==
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The biosynthesis of rebeccamycin, an antitumor compound, involves the, remarkable eight-electron oxidation of chlorinated chromopyrrolic acid., Although one rebeccamycin biosynthetic enzyme is capable of generating low, levels of the eight-electron oxidation product on its own, a second, protein, RebC, is required to accelerate product formation and eliminate, side reactions. However, the mode of action of RebC was largely unknown., Using crystallography, we have determined a likely function for RebC as a, flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding, pocket. These studies strongly suggest that the role of RebC is to, sequester a reactive intermediate produced by its partner protein and to, react with it enzymatically, preventing its conversion to a suite of, degradation products that includes, at low levels, the desired product.
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<StructureSection load='2r0g' size='340' side='right'caption='[[2r0g]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2r0g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R0G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7CK:7-CARBOXY-5-HYDROXY-12,13-DIHYDRO-6H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOLE'>7CK</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r0g OCA], [https://pdbe.org/2r0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r0g RCSB], [https://www.ebi.ac.uk/pdbsum/2r0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r0g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8KI25_LENAE Q8KI25_LENAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/2r0g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r0g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capable of generating low levels of the eight-electron oxidation product on its own, a second protein, RebC, is required to accelerate product formation and eliminate side reactions. However, the mode of action of RebC was largely unknown. Using crystallography, we have determined a likely function for RebC as a flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding pocket. These studies strongly suggest that the role of RebC is to sequester a reactive intermediate produced by its partner protein and to react with it enzymatically, preventing its conversion to a suite of degradation products that includes, at low levels, the desired product.
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==About this Structure==
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Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC.,Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:17873060<ref>PMID:17873060</ref>
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2R0G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=7CK:'>7CK</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R0G OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 14;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17873060 17873060]
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</div>
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[[Category: Lechevalieria aerocolonigenes]]
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<div class="pdbe-citations 2r0g" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Drennan, C.L.]]
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<references/>
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[[Category: Ryan, K.S.]]
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__TOC__
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[[Category: 7CK]]
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</StructureSection>
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[[Category: FAD]]
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[[Category: Large Structures]]
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[[Category: 7-carboxy-k252c]]
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[[Category: Lentzea aerocolonigenes]]
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[[Category: chromopyrrolic acid]]
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[[Category: Drennan CL]]
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[[Category: flavin adenine dinucleotide]]
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[[Category: Ryan KS]]
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[[Category: monooxygenase]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:30:10 2008''
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Current revision

Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c

PDB ID 2r0g

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