2xyz

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[[Image:2xyz.jpg|left|200px]]
 
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==De Novo model of Bacteriophage P22 virion coat protein==
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The line below this paragraph, containing "STRUCTURE_2xyz", creates the "Structure Box" on the page.
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<SX load='2xyz' size='340' side='right' viewer='molstar' caption='[[2xyz]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xyz]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XYZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xyz OCA], [https://pdbe.org/2xyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xyz RCSB], [https://www.ebi.ac.uk/pdbsum/2xyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xyz ProSAT]</span></td></tr>
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{{STRUCTURE_2xyz| PDB=2xyz | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_BPP22 CAPSD_BPP22] Assembles to form an icosahedral capsid with a T=7 symmetry.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. This process, conserved among dsDNA viruses such as herpes viruses and bacteriophages, is key to forming infectious virions. Bacteriophage P22 has served as a model system for this study in the past several decades. However, how capsid assembly is initiated, where and how scaffolding proteins bind to coat proteins in the procapsid, and the conformational changes upon capsid maturation still remain elusive. Here, we report Calpha backbone models for the P22 procapsid and infectious virion derived from electron cryomicroscopy density maps determined at 3.8- and 4.0-A resolution, respectively, and the first procapsid structure at subnanometer resolution without imposing symmetry. The procapsid structures show the scaffolding protein interacting electrostatically with the N terminus (N arm) of the coat protein through its C-terminal helix-loop-helix motif, as well as unexpected interactions between 10 scaffolding proteins and the 12-fold portal located at a unique vertex. These suggest a critical role for the scaffolding proteins both in initiating the capsid assembly at the portal vertex and propagating its growth on a T = 7 icosahedral lattice. Comparison of the procapsid and the virion backbone models reveals coordinated and complex conformational changes. These structural observations allow us to propose a more detailed molecular mechanism for the scaffolding-mediated capsid assembly initiation including portal incorporation, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.
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===DE NOVO MODEL OF BACTERIOPHAGE P22 VIRION COAT PROTEIN===
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Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus.,Chen DH, Baker ML, Hryc CF, Dimaio F, Jakana J, Wu W, Dougherty M, Haase-Pettingell C, Schmid MF, Jiang W, Baker D, King JA, Chiu W Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1355-60. Epub 2011 Jan 10. PMID:21220301<ref>PMID:21220301</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xyz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21220301}}, adds the Publication Abstract to the page
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21220301 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21220301}}
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__TOC__
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</SX>
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==About this Structure==
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[[Category: Large Structures]]
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[[2xyz]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XYZ OCA].
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[[Category: Salmonella virus P22]]
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[[Category: Baker D]]
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==Reference==
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[[Category: Baker ML]]
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<ref group="xtra">PMID:21220301</ref><references group="xtra"/>
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[[Category: Chen D-H]]
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[[Category: Enterobacteria phage p22]]
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[[Category: Chiu W]]
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[[Category: Baker, D.]]
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[[Category: DiMaio F]]
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[[Category: Baker, M L.]]
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[[Category: Dougherty M]]
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[[Category: Chen, D H.]]
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[[Category: Haase-Pettingell C]]
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[[Category: Chiu, W.]]
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[[Category: Hryc CF]]
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[[Category: Dimaio, F.]]
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[[Category: Jakana J]]
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[[Category: Dougherty, M.]]
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[[Category: Jiang W]]
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[[Category: Haase-Pettingell, C.]]
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[[Category: King JA]]
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[[Category: Hryc, C F.]]
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[[Category: Schmid MF]]
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[[Category: Jakana, J.]]
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[[Category: Wu W]]
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[[Category: Jiang, W.]]
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[[Category: King, J A.]]
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[[Category: Schmid, M F.]]
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[[Category: Wu, W.]]
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Current revision

De Novo model of Bacteriophage P22 virion coat protein

2xyz, resolution 4.00Å

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