3mqy

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[[Image:3mqy.png|left|200px]]
 
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==SgrAI with cleaved DNA and Magnesium bound==
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The line below this paragraph, containing "STRUCTURE_3mqy", creates the "Structure Box" on the page.
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<StructureSection load='3mqy' size='340' side='right'caption='[[3mqy]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3mqy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MQY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_3mqy| PDB=3mqy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mqy OCA], [https://pdbe.org/3mqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mqy RCSB], [https://www.ebi.ac.uk/pdbsum/3mqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mqy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9F6L0_STRGR Q9F6L0_STRGR]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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SgrAI is a type II restriction endonuclease that cuts an unusually long recognition sequence and exhibits allosteric self-activation with expansion of DNA-sequence specificity. The three-dimensional crystal structures of SgrAI bound to cleaved primary-site DNA and Mg(2)(+) and bound to secondary-site DNA with either Mg(2)(+) or Ca(2)(+) are presented. All three structures show a conformation of enzyme and DNA similar to the previously determined dimeric structure of SgrAI bound to uncleaved primary-site DNA and Ca(2)(+) [Dunten et al. (2008), Nucleic Acids Res. 36, 5405-5416], with the exception of the cleaved bond and a slight shifting of the DNA in the SgrAI/cleaved primary-site DNA/Mg(2)(+) structure. In addition, a new metal ion binding site is located in one of the two active sites in this structure, which is consistent with proposals for the existence of a metal-ion site near the 3'-O leaving group.
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===SgrAI with cleaved DNA and Magnesium bound===
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New clues in the allosteric activation of DNA cleavage by SgrAI: structures of SgrAI bound to cleaved primary-site DNA and uncleaved secondary-site DNA.,Little EJ, Dunten PW, Bitinaite J, Horton NC Acta Crystallogr D Biol Crystallogr. 2011 Jan;67(Pt 1):67-74. Epub 2010, Dec 16. PMID:21206063<ref>PMID:21206063</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21206063}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3mqy" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21206063 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21206063}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3mqy]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQY OCA].
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==Reference==
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<ref group="xtra">PMID:21206063</ref><references group="xtra"/>
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[[Category: Streptomyces griseus]]
[[Category: Streptomyces griseus]]
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[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Dunten PW]]
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[[Category: Dunten, P W.]]
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[[Category: Horton NC]]
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[[Category: Horton, N C.]]
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[[Category: Little EJ]]
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[[Category: Little, E J.]]
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Current revision

SgrAI with cleaved DNA and Magnesium bound

PDB ID 3mqy

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