2xiu

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[[Image:2xiu.jpg|left|200px]]
 
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==High resolution structure of MTSL-tagged CylR2.==
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The line below this paragraph, containing "STRUCTURE_2xiu", creates the "Structure Box" on the page.
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<StructureSection load='2xiu' size='340' side='right'caption='[[2xiu]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xiu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XIU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene></td></tr>
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{{STRUCTURE_2xiu| PDB=2xiu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xiu OCA], [https://pdbe.org/2xiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xiu RCSB], [https://www.ebi.ac.uk/pdbsum/2xiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xiu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8VL32_ENTFL Q8VL32_ENTFL]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Long-range structural information derived from paramagnetic relaxation enhancement observed in the presence of a paramagnetic nitroxide radical is highly useful for structural characterization of globular, modular and intrinsically disordered proteins, as well as protein-protein and protein-DNA complexes. Here we characterized the conformation of a spin-label attached to the homodimeric protein CylR2 using a combination of X-ray crystallography, electron paramagnetic resonance (EPR) and NMR spectroscopy. Close agreement was found between the conformation of the spin label observed in the crystal structure with interspin distances measured by EPR and signal broadening in NMR spectra, suggesting that the conformation seen in the crystal structure is also preferred in solution. In contrast, conformations of the spin label observed in crystal structures of T4 lysozyme are not in agreement with the paramagnetic relaxation enhancement observed for spin-labeled CylR2 in solution. Our data demonstrate that accurate positioning of the paramagnetic center is essential for high-resolution structure determination.
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===HIGH RESOLUTION STRUCTURE OF MTSL-TAGGED CYLR2.===
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Integrated analysis of the conformation of a protein-linked spin label by crystallography, EPR and NMR spectroscopy.,Gruene T, Cho MK, Karyagina I, Kim HY, Grosse C, Giller K, Zweckstetter M, Becker S J Biomol NMR. 2011 Jan 28. PMID:21271275<ref>PMID:21271275</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21271275}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2xiu" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21271275 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21271275}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2xiu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XIU OCA].
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==Reference==
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<ref group="xtra">PMID:21271275</ref><references group="xtra"/>
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[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
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[[Category: Becker, S.]]
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[[Category: Large Structures]]
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[[Category: Cho, M K.]]
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[[Category: Becker S]]
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[[Category: Giller, K.]]
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[[Category: Cho M-K]]
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[[Category: Grosse, C.]]
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[[Category: Giller K]]
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[[Category: Gruene, T.]]
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[[Category: Grosse C]]
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[[Category: Karyagina, I.]]
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[[Category: Gruene T]]
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[[Category: Kim, H Y.]]
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[[Category: Karyagina I]]
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[[Category: Zweckstetter, M.]]
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[[Category: Kim H-Y]]
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[[Category: Zweckstetter M]]

Current revision

High resolution structure of MTSL-tagged CylR2.

PDB ID 2xiu

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