1muc

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[[Image:1muc.gif|left|200px]]<br />
 
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<applet load="1muc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1muc, resolution 1.85&Aring;" />
 
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'''STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION==
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We report here the refined X-ray crystal structure of muconate lactonizing, enzyme (MLE) from Pseudomonas putida PRS2000 at a resolution of 1.85 A, with an R-factor of 16.8%. An enzyme from the beta-ketoadipate pathway, MLE catalyses the conversion of cis,cis-muconate to muconolactone. It is a, homo-octamer, one monomer consisting of 373 amino acid residues. MLE has, two large domains and a C-terminal subdomain: an alpha + beta domain, an, alpha beta-barrel domain and a C-terminal meandering subdomain. The alpha, beta-barrel domain is highly irregular. Its structure is (beta/alpha)7, beta, with the structural role of the last alpha-helix being replaced by, both the C-terminal subdomain and part of the N-terminal domain. The, fifth, seventh and eighth barrel strands are unusual because ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7500361 (full description)]]
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<StructureSection load='1muc' size='340' side='right'caption='[[1muc]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1muc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1mle 1mle]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MUC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MUC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1muc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1muc OCA], [https://pdbe.org/1muc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1muc RCSB], [https://www.ebi.ac.uk/pdbsum/1muc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1muc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CATB_PSEPU CATB_PSEPU] Catalyzes a syn cycloisomerization.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mu/1muc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1muc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1MUC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]] with MN as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1MLE. Active as [[http://en.wikipedia.org/wiki/Muconate_cycloisomerase Muconate cycloisomerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.1 5.5.1.1]]. Structure known Active Sites: MNA and MNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MUC OCA]].
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*[[Muconate cycloisomerase|Muconate cycloisomerase]]
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__TOC__
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==Reference==
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</StructureSection>
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The refined X-ray structure of muconate lactonizing enzyme from Pseudomonas putida PRS2000 at 1.85 A resolution., Helin S, Kahn PC, Guha BL, Mallows DG, Goldman A, J Mol Biol. 1995 Dec 15;254(5):918-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7500361 7500361]
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[[Category: Large Structures]]
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[[Category: Muconate cycloisomerase]]
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[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: Single protein]]
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[[Category: Goldman A]]
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[[Category: Goldman, A.]]
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[[Category: Guha BHL]]
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[[Category: Guha, B.H.L.]]
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[[Category: Helin S]]
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[[Category: Helin, S.]]
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[[Category: Kahn PC]]
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[[Category: Kahn, P.C.]]
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[[Category: Mallows DJ]]
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[[Category: Mallows, D.J.]]
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[[Category: MN]]
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[[Category: muconate lactonizing enzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:34:40 2007''
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Current revision

STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION

PDB ID 1muc

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