2qdf

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(New page: 200px<br /><applet load="2qdf" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qdf, resolution 2.200&Aring;" /> '''Structure of N-term...)
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[[Image:2qdf.jpg|left|200px]]<br /><applet load="2qdf" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qdf, resolution 2.200&Aring;" />
 
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'''Structure of N-terminal domain of E. Coli YaeT'''<br />
 
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==Overview==
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==Structure of N-terminal domain of E. Coli YaeT==
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Integral beta-barrel proteins are found in the outer membranes of, mitochondria, chloroplasts, and Gram-negative bacteria. The machine that, assembles these proteins contains an integral membrane protein, called, YaeT in Escherichia coli, which has one or more polypeptide, transport-associated (POTRA) domains. The crystal structure of a, periplasmic fragment of YaeT reveals the POTRA domain fold and suggests a, model for how POTRA domains can bind different peptide sequences, as, required for a machine that handles numerous beta-barrel protein, precursors. Analysis of POTRA domain deletions shows which are essential, and provides a view of the spatial organization of this assembly machine.
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<StructureSection load='2qdf' size='340' side='right'caption='[[2qdf]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qdf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QDF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qdf OCA], [https://pdbe.org/2qdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qdf RCSB], [https://www.ebi.ac.uk/pdbsum/2qdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qdf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BAMA_ECOLI BAMA_ECOLI] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamD, the core component of the assembly machinery.<ref>PMID:15951436</ref> <ref>PMID:16102012</ref> <ref>PMID:16824102</ref> <ref>PMID:20378773</ref> <ref>PMID:21823654</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qd/2qdf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qdf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Integral beta-barrel proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria. The machine that assembles these proteins contains an integral membrane protein, called YaeT in Escherichia coli, which has one or more polypeptide transport-associated (POTRA) domains. The crystal structure of a periplasmic fragment of YaeT reveals the POTRA domain fold and suggests a model for how POTRA domains can bind different peptide sequences, as required for a machine that handles numerous beta-barrel protein precursors. Analysis of POTRA domain deletions shows which are essential and provides a view of the spatial organization of this assembly machine.
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==About this Structure==
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Structure and function of an essential component of the outer membrane protein assembly machine.,Kim S, Malinverni JC, Sliz P, Silhavy TJ, Harrison SC, Kahne D Science. 2007 Aug 17;317(5840):961-4. PMID:17702946<ref>PMID:17702946</ref>
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2QDF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QDF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and function of an essential component of the outer membrane protein assembly machine., Kim S, Malinverni JC, Sliz P, Silhavy TJ, Harrison SC, Kahne D, Science. 2007 Aug 17;317(5840):961-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17702946 17702946]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2qdf" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Harrison, S.C.]]
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[[Category: Kahne, D.]]
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[[Category: Kim, S.]]
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[[Category: Malinverni, J.C.]]
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[[Category: Silhavy, T.J.]]
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[[Category: Sliz, P.]]
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[[Category: MG]]
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[[Category: membrane protein]]
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[[Category: potra domain]]
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[[Category: protein transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:46:25 2008''
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==See Also==
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*[[Bam complex 3D structures|Bam complex 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Harrison SC]]
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[[Category: Kahne D]]
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[[Category: Kim S]]
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[[Category: Malinverni JC]]
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[[Category: Silhavy TJ]]
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[[Category: Sliz P]]

Current revision

Structure of N-terminal domain of E. Coli YaeT

PDB ID 2qdf

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