2qdl

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(New page: 200px<br /><applet load="2qdl" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qdl, resolution 2.20&Aring;" /> '''Crystal structure of...)
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[[Image:2qdl.jpg|left|200px]]<br /><applet load="2qdl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qdl, resolution 2.20&Aring;" />
 
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'''Crystal structure of scaffolding protein TtCheW from Thermoanaerobacter tengcongensis'''<br />
 
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==Overview==
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==Crystal structure of scaffolding protein TtCheW from Thermoanaerobacter tengcongensis==
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The crystal structure of the scaffolding protein CheW from, Thermoanaerobacter tengcongensis (TtCheW) is reported with a resolution at, 2.2A using molecular replacement. Based on the crystal structure TmCheA, P4-P5-TmCheW from Thermotoga maritime reported by others, we modeled the, TmCheA P4-P5-TtCheW complex and predicted that TtCheW is involved in a, hydrophobic interaction with CheA, similar to that for TmCheW. We also, found that the conserved motif "NxxGxIxP" from CheW plays an important, role in CheA binding. The coincidence of the reported mutation sites, related to CheW-MCP binding, and the predicted protein interaction region, within the TtCheW molecule, suggest that CheW-MCP binding sites lie in the, groove-shaped area between TtCheW and the CheA P4 domain within the, assembled model.
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<StructureSection load='2qdl' size='340' side='right'caption='[[2qdl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qdl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QDL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qdl OCA], [https://pdbe.org/2qdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qdl RCSB], [https://www.ebi.ac.uk/pdbsum/2qdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qdl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8RBV8_CALS4 Q8RBV8_CALS4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qd/2qdl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qdl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the scaffolding protein CheW from Thermoanaerobacter tengcongensis (TtCheW) is reported with a resolution at 2.2A using molecular replacement. Based on the crystal structure TmCheA P4-P5-TmCheW from Thermotoga maritime reported by others, we modeled the TmCheA P4-P5-TtCheW complex and predicted that TtCheW is involved in a hydrophobic interaction with CheA, similar to that for TmCheW. We also found that the conserved motif "NxxGxIxP" from CheW plays an important role in CheA binding. The coincidence of the reported mutation sites related to CheW-MCP binding, and the predicted protein interaction region within the TtCheW molecule, suggest that CheW-MCP binding sites lie in the groove-shaped area between TtCheW and the CheA P4 domain within the assembled model.
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==About this Structure==
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Crystal structure of scaffolding protein CheW from thermoanaerobacter tengcongensis.,Yao W, Shi L, Liang DC Biochem Biophys Res Commun. 2007 Oct 5;361(4):1027-32. Epub 2007 Jul 31. PMID:17681283<ref>PMID:17681283</ref>
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2QDL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QDL OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of scaffolding protein CheW from thermoanaerobacter tengcongensis., Yao W, Shi L, Liang DC, Biochem Biophys Res Commun. 2007 Oct 5;361(4):1027-32. Epub 2007 Jul 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17681283 17681283]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2qdl" style="background-color:#fffaf0;"></div>
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[[Category: Thermoanaerobacter tengcongensis]]
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== References ==
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[[Category: Liang, D.C.]]
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<references/>
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[[Category: Shi, L.]]
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__TOC__
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[[Category: Yao, W.]]
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</StructureSection>
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[[Category: GOL]]
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
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[[Category: beta-barrel]]
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[[Category: Large Structures]]
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[[Category: signaling protein]]
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[[Category: Liang DC]]
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[[Category: Shi L]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:46:28 2008''
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[[Category: Yao W]]

Current revision

Crystal structure of scaffolding protein TtCheW from Thermoanaerobacter tengcongensis

PDB ID 2qdl

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