3a0k

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[[Image:3a0k.png|left|200px]]
 
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==Crystal structure of an antiflamatory legume lectin from Cymbosema roseum seeds==
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The line below this paragraph, containing "STRUCTURE_3a0k", creates the "Structure Box" on the page.
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<StructureSection load='3a0k' size='340' side='right'caption='[[3a0k]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3a0k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cymbosema_roseum Cymbosema roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A0K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_3a0k| PDB=3a0k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a0k OCA], [https://pdbe.org/3a0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a0k RCSB], [https://www.ebi.ac.uk/pdbsum/3a0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a0k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D5MNX4_CYMRO D5MNX4_CYMRO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a0/3a0k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a0k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection and anti-inflammatory effects when by systemic injection. Protein sequencing was obtained by Tandem Mass Spectrometry and the crystal structure was solved by X-ray crystallography using a Synchrotron radiation source. Molecular replacement and refinement were performed using CCP4 and the carbohydrate binding properties were described by affinity assays and computational docking. Biological assays were performed in order to evaluate the lectin edematogenic activity. The crystal structure of CRLI was established to a 1.8A resolution in order to determine a structural basis for these differing activities. The structure of CRLI is closely homologous to those of other legume lectins at the monomer level and assembles into tetramers as do many of its homologues. The CRLI carbohydrate binding site was predicted by docking with a specific inhibitory trisaccharide. CRLI possesses a hydrophobic pocket for the binding of alpha-aminobutyric acid and that pocket is occupied in this structure as are the binding sites for calcium and manganese cations characteristic of legume lectins. CRLI route-dependent effects for acute inflammation are related to its carbohydrate binding domain (due to inhibition caused by the presence of alpha-methyl-mannoside), and are based on comparative analysis with ConA crystal structure. This may be due to carbohydrate binding site design, which differs at Tyr12 and Glu205 position.
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===Crystal structure of an antiflamatory legume lectin from Cymbosema roseum seeds===
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Structural basis for both pro- and anti-inflammatory response induced by mannose-specific legume lectin from Cymbosema roseum.,Rocha BA, Delatorre P, Oliveira TM, Benevides RG, Pires AF, Sousa AA, Souza LA, Assreuy AM, Debray H, de Azevedo WF Jr, Sampaio AH, Cavada BS Biochimie. 2011 Jan 26. PMID:21277932<ref>PMID:21277932</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21277932}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3a0k" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21277932 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21277932}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3a0k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cymbosema_roseum Cymbosema roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A0K OCA].
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==Reference==
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<ref group="xtra">PMID:21277932</ref><references group="xtra"/>
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[[Category: Cymbosema roseum]]
[[Category: Cymbosema roseum]]
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[[Category: Benevides, R G.]]
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[[Category: Large Structures]]
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[[Category: Cavada, B S.]]
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[[Category: Benevides RG]]
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[[Category: Delatorre, P.]]
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[[Category: Cavada BS]]
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[[Category: Marinho, E S.]]
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[[Category: Delatorre P]]
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[[Category: Moura, T R.]]
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[[Category: Marinho ES]]
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[[Category: Nascimento, K S.]]
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[[Category: Moura TR]]
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[[Category: Rocha, B A.M.]]
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[[Category: Nascimento KS]]
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[[Category: Sampaio, A H.]]
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[[Category: Rocha BAM]]
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[[Category: Souza, L A.G.]]
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[[Category: Sampaio AH]]
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[[Category: Souza LAG]]

Current revision

Crystal structure of an antiflamatory legume lectin from Cymbosema roseum seeds

PDB ID 3a0k

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OCA

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