3o39

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[[Image:3o39.jpg|left|200px]]
 
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==Crystal Structure of SPY==
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The line below this paragraph, containing "STRUCTURE_3o39", creates the "Structure Box" on the page.
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<StructureSection load='3o39' size='340' side='right'caption='[[3o39]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3o39]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._EDL933 Escherichia coli O157:H7 str. EDL933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O39 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.599&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3o39| PDB=3o39 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o39 OCA], [https://pdbe.org/3o39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o39 RCSB], [https://www.ebi.ac.uk/pdbsum/3o39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o39 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPY_ECO57 SPY_ECO57] An ATP-independent periplasmic chaperone, decreases protein aggregation and helps protein refolding. Binds substrate over a large region. Protect proteins in vitro against tannin inactivation; tannins have antimicrobial activity. Overexpression enhances the stability of otherwise unstable periplasmic proteins (PubMed:21317898).<ref>PMID:21317898</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To optimize the in vivo folding of proteins, we linked protein stability to antibiotic resistance, thereby forcing bacteria to effectively fold and stabilize proteins. When we challenged Escherichia coli to stabilize a very unstable periplasmic protein, it massively overproduced a periplasmic protein called Spy, which increases the steady-state levels of a set of unstable protein mutants up to 700-fold. In vitro studies demonstrate that the Spy protein is an effective ATP-independent chaperone that suppresses protein aggregation and aids protein refolding. Our strategy opens up new routes for chaperone discovery and the custom tailoring of the in vivo folding environment. Spy forms thin, apparently flexible cradle-shaped dimers. The structure of Spy is unlike that of any previously solved chaperone, making it the prototypical member of a new class of small chaperones that facilitate protein refolding in the absence of energy cofactors.
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===Crystal Structure of SPY===
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Genetic selection designed to stabilize proteins uncovers a chaperone called Spy.,Quan S, Koldewey P, Tapley T, Kirsch N, Ruane KM, Pfizenmaier J, Shi R, Hofmann S, Foit L, Ren G, Jakob U, Xu Z, Cygler M, Bardwell JC Nat Struct Mol Biol. 2011 Feb 13. PMID:21317898<ref>PMID:21317898</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3o39]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O39 OCA].
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<div class="pdbe-citations 3o39" style="background-color:#fffaf0;"></div>
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[[Category: Escherichia coli]]
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== References ==
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[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
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<references/>
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[[Category: Cygler, M.]]
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__TOC__
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[[Category: Ruane, K M.]]
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</StructureSection>
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[[Category: Shi, R.]]
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[[Category: Escherichia coli O157:H7 str. EDL933]]
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[[Category: Large Structures]]
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[[Category: Cygler M]]
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[[Category: Ruane KM]]
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[[Category: Shi R]]

Current revision

Crystal Structure of SPY

PDB ID 3o39

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