3p71

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[[Image:3p71.jpg|left|200px]]
 
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==Crystal structure of the complex of LCMT-1 and PP2A==
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<StructureSection load='3p71' size='340' side='right'caption='[[3p71]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3p71]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P71 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AN6:5-{[(3S)-3-AMINO-3-CARBOXYPROPYL](ETHYL)AMINO}-5-DEOXYADENOSINE'>AN6</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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{{STRUCTURE_3p71| PDB=3p71 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p71 OCA], [https://pdbe.org/3p71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p71 RCSB], [https://www.ebi.ac.uk/pdbsum/3p71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p71 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PP2AA_HUMAN PP2AA_HUMAN] PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.<ref>PMID:9920888</ref> <ref>PMID:10801873</ref> <ref>PMID:22613722</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proper formation of protein phosphatase 2A (PP2A) holoenzymes is essential for the fitness of all eukaryotic cells. Carboxyl methylation of the PP2A catalytic subunit plays a critical role in regulating holoenzyme assembly; methylation is catalyzed by PP2A-specific methyltransferase LCMT-1, an enzyme required for cell survival. We determined crystal structures of human LCMT-1 in isolation and in complex with PP2A stabilized by a cofactor mimic. The structures show that the LCMT-1 active-site pocket recognizes the carboxyl terminus of PP2A, and, interestingly, the PP2A active site makes extensive contacts to LCMT-1. We demonstrated that activation of the PP2A active site stimulates methylation, suggesting a mechanism for efficient conversion of activated PP2A into substrate-specific holoenzymes, thus minimizing unregulated phosphatase activity or formation of inactive holoenzymes. A dominant-negative LCMT-1 mutant attenuates the cell cycle without causing cell death, likely by inhibiting uncontrolled phosphatase activity. Our studies suggested mechanisms of LCMT-1 in tight control of PP2A function, important for the cell cycle and cell survival.
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===Crystal structure of the complex of LCMT-1 and PP2A===
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The Structural Basis for Tight Control of PP2A Methylation and Function by LCMT-1.,Stanevich V, Jiang L, Satyshur KA, Li Y, Jeffrey PD, Li Z, Menden P, Semmelhack MF, Xing Y Mol Cell. 2011 Feb 4;41(3):331-42. PMID:21292165<ref>PMID:21292165</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3p71" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21292165 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21292165}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3p71]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P71 OCA].
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==Reference==
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<ref group="xtra">PMID:21292165</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphoprotein phosphatase]]
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[[Category: Large Structures]]
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[[Category: Jiang, L]]
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[[Category: Jiang L]]
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[[Category: Satyshur, K A.]]
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[[Category: Satyshur KA]]
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[[Category: Stanevich, V.]]
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[[Category: Stanevich V]]
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[[Category: Xing, Y.]]
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[[Category: Xing Y]]

Current revision

Crystal structure of the complex of LCMT-1 and PP2A

PDB ID 3p71

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