1yas

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[[Image:1yas.gif|left|200px]]<br />
 
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<applet load="1yas" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1yas, resolution 1.9&Aring;" />
 
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'''HYDROXYNITRILE LYASE COMPLEXED WITH HISTIDINE'''<br />
 
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==Overview==
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==HYDROXYNITRILE LYASE COMPLEXED WITH HISTIDINE==
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BACKGROUND: Over three thousand species of plants, including important, food crops such as cassava, use cyanogenesis, the liberation of HCN upon, tissue damage, as a defense against predation. Detoxification of, cyanogenic food crops requires disruption of the cyanogenic pathway., Hydroxynitrile lyase is one of the key enzymes in cyanogenesis, catalyzing, the decomposition of an alpha-cyanohydrin to form HCN plus the, corresponding aldehyde or ketone. These enzymes are also of potential, utility for industrial syntheses of optically pure chiral cyanohydrins, being used to catalyze the reverse reaction. We set out to gain insight, into the catalytic mechanism of this important class of enzymes by, determining the three-dimensional structure of hydroxynitrile lyase from, the rubber tree, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8805565 (full description)]]
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<StructureSection load='1yas' size='340' side='right'caption='[[1yas]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1yas]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YAS FirstGlance]. <br>
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1YAS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]] with SO4 and HIS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transfered_to_EC_4.1.2.37 Transfered to EC 4.1.2.37]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.39 4.1.2.39]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YAS OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yas OCA], [https://pdbe.org/1yas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yas RCSB], [https://www.ebi.ac.uk/pdbsum/1yas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yas ProSAT]</span></td></tr>
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Mechanism of cyanogenesis: the crystal structure of hydroxynitrile lyase from Hevea brasiliensis., Wagner UG, Hasslacher M, Griengl H, Schwab H, Kratky C, Structure. 1996 Jul 15;4(7):811-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8805565 8805565]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HNL_HEVBR HNL_HEVBR] Involved in cyanogenesis, the release of HCN from injured tissues. Decomposes a varieties of (R) or (S) cyanohydrins into HCN and the corresponding aldehydes and ketones. The natural substrate of this enzyme is (S)-acetone cyanohydrin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ya/1yas_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yas ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Hevea brasiliensis]]
[[Category: Hevea brasiliensis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Transfered to EC 4.1.2.37]]
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[[Category: Kratky C]]
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[[Category: Kratky, C.]]
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[[Category: Wagner UG]]
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[[Category: Wagner, U.G.]]
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[[Category: HIS]]
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[[Category: SO4]]
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[[Category: complex (lyase/peptide)]]
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[[Category: cyanhydrin formation]]
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[[Category: cyanogenesis]]
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[[Category: lyase]]
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[[Category: oxynitrilase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:36:22 2007''
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Current revision

HYDROXYNITRILE LYASE COMPLEXED WITH HISTIDINE

PDB ID 1yas

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