2ewv

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(New page: 200px<br /><applet load="2ewv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ewv, resolution 2.8&Aring;" /> '''Crystal Structure of ...)
Current revision (09:20, 14 February 2024) (edit) (undo)
 
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[[Image:2ewv.gif|left|200px]]<br /><applet load="2ewv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ewv, resolution 2.8&Aring;" />
 
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'''Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP'''<br />
 
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==Overview==
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==Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP==
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PilT is a hexameric ATPase required for bacterial type IV pilus retraction, and surface motility. Crystal structures of ADP- and ATP-bound Aquifex, aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and, C-terminal RecA-like ATPase domains followed by a set of short C-terminal, helices. The hexamer is formed by extensive polar subunit interactions, between the ATPase core of one monomer and the N-terminal domain of the, next. An additional structure captures a nonsymmetric PilT hexamer in, which approach of invariant arginines from two subunits to the bound, nucleotide forms an enzymatically competent active site. A panel of pilT, mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We, present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this, hexameric motor. Our conclusions apply to the entire type II/IV secretion, ATPase family.
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<StructureSection load='2ewv' size='340' side='right'caption='[[2ewv]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2ewv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EWV FirstGlance]. <br>
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2EWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5] with <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ewv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ewv OCA], [https://pdbe.org/2ewv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ewv RCSB], [https://www.ebi.ac.uk/pdbsum/2ewv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ewv ProSAT]</span></td></tr>
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Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility., Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT, Structure. 2007 Mar;15(3):363-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17355871 17355871]
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</table>
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[[Category: Aquifex aeolicus vf5]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/O66950_AQUAE O66950_AQUAE]
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[[Category: Forest, K.T.]]
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== Evolutionary Conservation ==
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[[Category: Satyshur, K.A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: ADP]]
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Check<jmol>
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[[Category: atpase]]
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<jmolCheckbox>
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[[Category: hexameric pilt]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/2ewv_consurf.spt"</scriptWhenChecked>
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[[Category: pilus retraction motor]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:55:18 2008''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ewv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Aquifex aeolicus VF5]]
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[[Category: Large Structures]]
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[[Category: Forest KT]]
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[[Category: Satyshur KA]]

Current revision

Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP

PDB ID 2ewv

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