2y95
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2y95 is ON HOLD Authors: Duszczyk, M.M., Sattler, M. Description: Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats es...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation== | |
| + | <StructureSection load='2y95' size='340' side='right'caption='[[2y95]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2y95]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y95 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y95 OCA], [https://pdbe.org/2y95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y95 RCSB], [https://www.ebi.ac.uk/pdbsum/2y95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y95 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors. | ||
| - | + | The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization.,Duszczyk MM, Wutz A, Rybin V, Sattler M RNA. 2011 Nov;17(11):1973-82. Epub 2011 Sep 26. PMID:21947263<ref>PMID:21947263</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2y95" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Duszczyk MM]] | ||
| + | [[Category: Sattler M]] | ||
Current revision
Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation
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