1gow

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[[Image:1gow.gif|left|200px]]<br />
 
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<applet load="1gow" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gow, resolution 2.6&Aring;" />
 
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'''BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS'''<br />
 
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==Overview==
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==BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS==
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Enzymes from hyperthermophilic organisms must operate at temperatures, which rapidly denature proteins from mesophiles. The structural basis of, this thermostability is still poorly understood. Towards a further, understanding of hyperthermostability, we have determined the crystal, structure of the beta-glycosidase (clan GH-1A, family 1) from the, hyperthermophilic archaeon Sulfolobus solfataricus at 2.6 A resolution., The enzyme is a tetramer with subunit molecular mass at 60 kDa, and, crystallises with half of the tetramer in the asymmetric unit. The, structure is a (betaalpha)8 barrel, but with substantial elaborations, between the beta-strands and alpha-helices in each repeat. The active site, occurs at the centre of the top face of the barrel and is connected to the, surface by a ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9299327 (full description)]]
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<StructureSection load='1gow' size='340' side='right'caption='[[1gow]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gow]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GOW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gow OCA], [https://pdbe.org/1gow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gow RCSB], [https://www.ebi.ac.uk/pdbsum/1gow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gow ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BGAL_SACS2 BGAL_SACS2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1gow_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gow ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GOW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]]. Active as [[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23]]. Structure known Active Sites: CTA and CTB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GOW OCA]].
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*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability., Aguilar CF, Sanderson I, Moracci M, Ciaramella M, Nucci R, Rossi M, Pearl LH, J Mol Biol. 1997 Sep 5;271(5):789-802. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9299327 9299327]
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[[Category: Large Structures]]
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[[Category: Beta-galactosidase]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Single protein]]
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[[Category: Aguilar CF]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Pearl LH]]
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[[Category: Aguilar, C.F.]]
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[[Category: Sanderson I]]
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[[Category: Pearl, L.H.]]
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[[Category: Sanderson, I.]]
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[[Category: beta-glycosidase]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:37:17 2007''
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Current revision

BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS

PDB ID 1gow

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