2y9z
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2y9z is ON HOLD until sometime in the future Authors: Yamada, K., Frouws, T.D., Angst, B., Fitzgerald, D.J., DeLuca, C., Schimmele, K., Sargent, D.F...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Chromatin Remodeling Factor ISW1a(del_ATPase) in DNA complex== | |
| + | <StructureSection load='2y9z' size='340' side='right'caption='[[2y9z]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2y9z]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y9Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.601Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9z OCA], [https://pdbe.org/2y9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y9z RCSB], [https://www.ebi.ac.uk/pdbsum/2y9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y9z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ISW1_YEAST ISW1_YEAST] Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.<ref>PMID:10090725</ref> <ref>PMID:11238381</ref> <ref>PMID:14622597</ref> <ref>PMID:12482963</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Site-specific recognition of DNA in eukaryotic organisms depends on the arrangement of nucleosomes in chromatin. In the yeast Saccharomyces cerevisiae, ISW1a and related chromatin remodelling factors are implicated in establishing the nucleosome repeat during replication and altering nucleosome position to affect gene activity. Here we have solved the crystal structures of S. cerevisiae ISW1a lacking its ATPase domain both alone and with DNA bound at resolutions of 3.25 A and 3.60 A, respectively, and we have visualized two different nucleosome-containing remodelling complexes using cryo-electron microscopy. The composite X-ray and electron microscopy structures combined with site-directed photocrosslinking analyses of these complexes suggest that ISW1a uses a dinucleosome substrate for chromatin remodelling. Results from a remodelling assay corroborate the dinucleosome model. We show how a chromatin remodelling factor could set the spacing between two adjacent nucleosomes acting as a 'protein ruler'. | ||
| - | + | Structure and mechanism of the chromatin remodelling factor ISW1a.,Yamada K, Frouws TD, Angst B, Fitzgerald DJ, DeLuca C, Schimmele K, Sargent DF, Richmond TJ Nature. 2011 Apr 28;472(7344):448-53. PMID:21525927<ref>PMID:21525927</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2y9z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Angst B]] | ||
| + | [[Category: DeLuca C]] | ||
| + | [[Category: Fitzgerald DJ]] | ||
| + | [[Category: Frouws TD]] | ||
| + | [[Category: Richmond TJ]] | ||
| + | [[Category: Sargent DF]] | ||
| + | [[Category: Schimmele K]] | ||
| + | [[Category: Yamada K]] | ||
Current revision
Chromatin Remodeling Factor ISW1a(del_ATPase) in DNA complex
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