2xti

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[[Image:2xti.png|left|200px]]
 
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==Asparaginyl-tRNA synthetase from Brugia malayi complexed with ATP:Mg and L-Asp-beta-NOH adenylate:PPi:Mg==
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The line below this paragraph, containing "STRUCTURE_2xti", creates the "Structure Box" on the page.
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<StructureSection load='2xti' size='340' side='right'caption='[[2xti]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xti]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XTI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NB8:5-O-[(R)-{[(2S)-2-AMINO-4-(HYDROXYAMINO)-4-OXOBUTANOYL]OXY}(HYDROXY)PHOSPHORYL]ADENOSINE'>NB8</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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{{STRUCTURE_2xti| PDB=2xti | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xti OCA], [https://pdbe.org/2xti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xti RCSB], [https://www.ebi.ac.uk/pdbsum/2xti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xti ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aminoacyl-tRNA synthetases are validated molecular targets for anti-infective drug discovery because of their essentiality in protein synthesis. Thanks to genome sequencing, it is now possible to systematically study aminoacyl-tRNA synthetases from human eukaryotic parasites as putative targets for novel drug discovery. As part of a program targeting class IIb asparaginyl-tRNA synthetases (AsnRS) from the parasitic nematode Brugia malayi for anti-filarial drugs, we report the complete structure of a eukaryotic AsnRS. Metazoan and fungal AsnRS differ from their bacterial homologues by the addition of a conserved N-terminal extension of about 110 residues whose structure we have determined by solution NMR for the B. malayi enzyme. In addition, we solved by X-ray crystallography a series of structures of the catalytically active N-terminally truncated enzyme (residues 112-548), allowing the structural basis for the mechanism of asparagine activation to be elucidated. The N-terminal domain contains a structured region with a novel fold featuring a lysine-rich helix that is shown by NMR to interact with tRNA. This is connected by an unstructured tether to the remainder of the enzyme, which is highly similar to the known structure of bacterial AsnRS. These data enable a model of the complete AsnRS-tRNA complex to be constructed.
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===ASPARAGINYL-TRNA SYNTHETASE FROM BRUGIA MALAYI COMPLEXED WITH ATP:MG AND L-ASP-BETA-NOH ADENYLATE:PPI:MG===
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A Hybrid Structural Model of the Complete Brugia malayi Cytoplasmic Asparaginyl-tRNA Synthetase.,Crepin T, Peterson F, Haertlein M, Jensen D, Wang C, Cusack S, Kron M J Mol Biol. 2010 Dec 4. PMID:21134380<ref>PMID:21134380</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xti" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21134380}}, adds the Publication Abstract to the page
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21134380 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21134380}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2xti]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XTI OCA].
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==Reference==
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<ref group="xtra">PMID:21134380</ref><ref group="xtra">PMID:16645791</ref><references group="xtra"/>
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[[Category: Brugia malayi]]
[[Category: Brugia malayi]]
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[[Category: Leucine--tRNA ligase]]
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[[Category: Large Structures]]
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[[Category: Crepin, T.]]
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[[Category: Crepin T]]
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[[Category: Cusack, S.]]
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[[Category: Cusack S]]
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[[Category: Haertlein, M.]]
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[[Category: Haertlein M]]
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[[Category: Kron, M.]]
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[[Category: Kron M]]

Current revision

Asparaginyl-tRNA synthetase from Brugia malayi complexed with ATP:Mg and L-Asp-beta-NOH adenylate:PPi:Mg

PDB ID 2xti

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