3ovp

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[[Image:3ovp.jpg|left|200px]]
 
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==Crystal Structure of hRPE==
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The line below this paragraph, containing "STRUCTURE_3ovp", creates the "Structure Box" on the page.
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<StructureSection load='3ovp' size='340' side='right'caption='[[3ovp]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ovp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OVP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OVP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.695&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=XPE:3,6,9,12,15,18,21,24,27-NONAOXANONACOSANE-1,29-DIOL'>XPE</scene></td></tr>
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{{STRUCTURE_3ovp| PDB=3ovp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ovp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ovp OCA], [https://pdbe.org/3ovp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ovp RCSB], [https://www.ebi.ac.uk/pdbsum/3ovp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ovp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPE_HUMAN RPE_HUMAN] Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.<ref>PMID:20923965</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The pentose phosphate pathway (PPP) confers protection against oxidative stress by supplying NADPH necessary for the regeneration of glutathione, which detoxifies H(2)O(2) into H(2)O and O(2). RPE functions in the PPP, catalyzing the reversible conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate and is an important enzyme for cellular response against oxidative stress. Here, using structural, biochemical, and functional studies, we show that human D-ribulose 5-phosphate 3-epimerase (hRPE) uses Fe(2+) for catalysis. Structures of the binary complexes of hRPE with D-ribulose 5-phosphate and D-xylulose 5-phosphate provide the first detailed molecular insights into the binding mode of physiological ligands and reveal an octahedrally coordinated Fe(2+) ion buried deep inside the active site. Human RPE folds into a typical (beta/alpha)(8) triosephosphate isomerase (TIM) barrel with a loop regulating access to the active site. Two aspartic acids are well positioned to carry out the proton transfers in an acid-base type of reaction mechanism. Interestingly, mutating Ser-10 to alanine almost abolished the enzymatic activity, while L12A and M72A mutations resulted in an almost 50% decrease in the activity. The binary complexes of hRPE reported here will aid in the design of small molecules for modulating the activity of the enzyme and altering flux through the PPP.
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===Crystal Structure of hRPE===
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Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE.,Liang W, Ouyang S, Shaw N, Joachimiak A, Zhang R, Liu ZJ FASEB J. 2011 Feb;25(2):497-504. Epub 2010 Oct 5. PMID:20923965<ref>PMID:20923965</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ovp" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20923965 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20923965}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3ovp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OVP OCA].
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==Reference==
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<ref group="xtra">PMID:20923965</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Ribulose-phosphate 3-epimerase]]
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[[Category: Large Structures]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Liang, W G.]]
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[[Category: Liang WG]]
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[[Category: Liu, Z J.]]
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[[Category: Liu ZJ]]
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[[Category: Ouyang, S Y.]]
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[[Category: Ouyang SY]]
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[[Category: Shaw, N.]]
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[[Category: Shaw N]]
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[[Category: Zhang, R G.]]
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[[Category: Zhang RG]]

Current revision

Crystal Structure of hRPE

PDB ID 3ovp

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