Sandbox CsrA

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{{Template:Checkbox Toggle CsrRNABinding}}
{{Template:Checkbox Toggle CsrRNABinding}}
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==Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine"> PMID:16923806 </ref>==
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===Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine"> PMID:16923806 </ref>===
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr28}}
{{Template:Checkbox Toggle Csr28}}
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{{Template:Checkbox Toggle CsrRNABinding}}
{{Template:Checkbox Toggle CsrRNABinding}}
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==Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />==
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===Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />===
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr28}}
{{Template:Checkbox Toggle Csr28}}
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==Aligned CsrA structures==
==Aligned CsrA structures==
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<Structure load='Image:1y00model1.pdb' size='500' frame='true' align='right' caption='CsRA structures aligned' scene='Sandbox_CsrA/Csrapdbs/3' />
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<Structure load='Image:1y00model1.pdb' size='500' frame='true' align='right' caption='CsRA structures aligned' scene='Sandbox_CsrA/Csrapdbs/6' />
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Display <scene name='Sandbox_CsrA/Csrapdbs/4'>backbone trace of aligned CsrA structures</scene>.
 
{{Template:Checkbox Toggle CsrRNABinding}}
{{Template:Checkbox Toggle CsrRNABinding}}
-
==Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />==
+
===Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />===
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr28}}
{{Template:Checkbox Toggle Csr28}}
 +
===Aligned Structures===
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Display <scene name='Sandbox_CsrA/Csrapdbs/6'>cartoon representation of aligned CsrA structures</scene>.<br>
 +
{{Template:Checkbox Toggle Model1Cartoon}} (<font color="cyan">'''E. coli'''</font>) <br>
 +
{{Template:Checkbox Toggle Model2Cartoon}} (<font color="pink">'''Pseudomonas aeruginosa'''</font>)<br>
 +
{{Template:Checkbox Toggle Model3Cartoon}} (<font color="wheat">'''Yersinia enterocolitica'''</font>)<br>
 +
&nbsp;
-
 
+
Display <scene name='Sandbox_CsrA/Csrapdbs/5'>backbone trace of aligned CsrA structures</scene>.<br>
 +
{{Template:Checkbox Toggle Model1BackboneTrace}} (<font color="cyan">'''E. coli'''</font>) <br>
 +
{{Template:Checkbox Toggle Model2BackboneTrace}} (<font color="pink">'''Pseudomonas aeruginosa'''</font>)<br>
 +
{{Template:Checkbox Toggle Model3BackboneTrace}} (<font color="wheat">'''Yersinia enterocolitica'''</font>)<br>
&nbsp;
&nbsp;
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&nbsp;
&nbsp;
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==Model of Vibrio CsrA==
==Model of Vibrio CsrA==
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The protein structure described in this section was determined [[theoretical models|theoretically]], and hence should be interpreted with caution.</td></tr></table>
The protein structure described in this section was determined [[theoretical models|theoretically]], and hence should be interpreted with caution.</td></tr></table>
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<Structure load='Image:VfischeriCsrA2dimer.pdb' size='500' frame='true' align='right' caption='CsRA (E. coli)' scene='Sandbox_CsrA/Vibriomodel/1' />
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<Structure load='Image:VfischeriCsrA2dimer.pdb' size='500' frame='true' align='right' caption='CsRA (V. fischeri)' scene='Sandbox_CsrA/Vibriomodel/1' />
{{Template:Checkbox Toggle CsrRNABinding}}
{{Template:Checkbox Toggle CsrRNABinding}}
-
==Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />==
+
===Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />===
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr28}}
{{Template:Checkbox Toggle Csr28}}
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<table style="background-color:#ffffc0" cellpadding="8" width="95%" border="0"><tr><td valign='top'>[[Image:Note.gif|left]] '''Theoretical Model:'''
<table style="background-color:#ffffc0" cellpadding="8" width="95%" border="0"><tr><td valign='top'>[[Image:Note.gif|left]] '''Theoretical Model:'''
The ''Vibrio'' structure described in this section was determined [[theoretical models|theoretically]], and hence should be interpreted with caution.</td></tr></table>
The ''Vibrio'' structure described in this section was determined [[theoretical models|theoretically]], and hence should be interpreted with caution.</td></tr></table>
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<Structure load='1vpz' size='500' frame='true' align='right' caption='CsRA structures aligned' scene='Sandbox_CsrA/Structuresplusmodel/3' />
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<Structure load='1vpz' size='500' frame='true' align='right' caption='CsRA structures aligned' scene='Sandbox_CsrA/Structuresplusmodel/5' />
-
 
+
-
Display <scene name='Sandbox_CsrA/Structuresplusmodel/4'>backbone trace of aligned structures and model</scene>.
+
-
 
+
{{Template:Checkbox Toggle CsrRNABinding}}
{{Template:Checkbox Toggle CsrRNABinding}}
-
==Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />==
+
===Residues Highlighted by Alanine Scanning Mutation of CsrA<ref name="Alanine" />===
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr19}}<br>
{{Template:Checkbox Toggle Csr28}}
{{Template:Checkbox Toggle Csr28}}
 +
===Aligned Structures===
 +
Display <scene name='Sandbox_CsrA/Structuresplusmodel/5'>cartoon representation of aligned CsrA structures</scene>.<br>
 +
{{Template:Checkbox Toggle Model1Cartoon}} (<font color="cyan">'''E. coli'''</font>) <br>
 +
{{Template:Checkbox Toggle Model2Cartoon}} (<font color="pink">'''Pseudomonas aeruginosa'''</font>)<br>
 +
{{Template:Checkbox Toggle Model3Cartoon}} (<font color="wheat">'''Yersinia enterocolitica'''</font>)<br>
 +
{{Template:Checkbox Toggle Model4Cartoon}} (<font color="magenta">'''Vibrio fischeri'''</font>)<br>
 +
 +
&nbsp;
 +
Display <scene name='Sandbox_CsrA/Structuresplusmodel/6'>backbone trace of aligned CsrA structures</scene>.<br>
 +
{{Template:Checkbox Toggle Model1BackboneTrace}} (<font color="cyan">'''E. coli'''</font>) <br>
 +
{{Template:Checkbox Toggle Model2BackboneTrace}} (<font color="pink">'''Pseudomonas aeruginosa'''</font>)<br>
 +
{{Template:Checkbox Toggle Model3BackboneTrace}} (<font color="wheat">'''Yersinia enterocolitica'''</font>)<br>
 +
{{Template:Checkbox Toggle Model4BackboneTrace}} (<font color="magenta">'''Vibrio fischeri'''</font>)<br>

Current revision

Contents

CsrA

CsRA (Pseudomonas aeruginosa)

Drag the structure with the mouse to rotate

Residues 24-27 form a [1].



Residues Highlighted by Alanine Scanning Mutation of CsrA[2]





 


 


 


 


 


 


 


 


E. coli CsrA

CsRA (E. coli)

Drag the structure with the mouse to rotate


Residues Highlighted by Alanine Scanning Mutation of CsrA[2]





 


 


 


 


 


 


 


 


Aligned CsrA structures

CsRA structures aligned

Drag the structure with the mouse to rotate




Residues Highlighted by Alanine Scanning Mutation of CsrA[2]



Aligned Structures

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)

 

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)
 


 


 


 


 


 


 


 

Model of Vibrio CsrA

Theoretical Model: The protein structure described in this section was determined theoretically, and hence should be interpreted with caution.

CsRA (V. fischeri)

Drag the structure with the mouse to rotate


Residues Highlighted by Alanine Scanning Mutation of CsrA[2]





 


 


 


 


 


 


 


 



Aligned CsrA structures and Vibrio Model

Theoretical Model: The Vibrio structure described in this section was determined theoretically, and hence should be interpreted with caution.

CsRA structures aligned

Drag the structure with the mouse to rotate


Residues Highlighted by Alanine Scanning Mutation of CsrA[2]



Aligned Structures

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)

(Vibrio fischeri)

 

Display .

(E. coli)

(Pseudomonas aeruginosa)

(Yersinia enterocolitica)

(Vibrio fischeri)


Structures Used On This Page

1y00 is a 2 chains structure of sequences from Escherichia coli. Full experimental information is available from OCA.

1vpz is a 2 chains structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

2bti is a 2 chains structure of sequences from Yersinia enterocolitica. Full crystallographic information is available from OCA.

See Also

1t3o - NMR structure of Bacillus subtilis CsrA

References

  1. ↑ Rife C, Schwarzenbacher R, McMullan D, Abdubek P, Ambing E, Axelrod H, Biorac T, Canaves JM, Chiu HJ, Deacon AM, DiDonato M, Elsliger MA, Godzik A, Grittini C, Grzechnik SK, Hale J, Hampton E, Han GW, Haugen J, Hornsby M, Jaroszewski L, Klock HE, Koesema E, Kreusch A, Kuhn P, Lesley SA, Miller MD, Moy K, Nigoghossian E, Paulsen J, Quijano K, Reyes R, Sims E, Spraggon G, Stevens RC, van den Bedem H, Velasquez J, Vincent J, White A, Wolf G, Xu Q, Hodgson KO, Wooley J, Wilson IA. Crystal structure of the global regulatory protein CsrA from Pseudomonas putida at 2.05 A resolution reveals a new fold. Proteins. 2005 Nov 1;61(2):449-53. PMID:16104018 doi:10.1002/prot.20502
  2. ↑ 2.0 2.1 2.2 2.3 2.4 Mercante J, Suzuki K, Cheng X, Babitzke P, Romeo T. Comprehensive alanine-scanning mutagenesis of Escherichia coli CsrA defines two subdomains of critical functional importance. J Biol Chem. 2006 Oct 20;281(42):31832-42. Epub 2006 Aug 21. PMID:16923806 doi:10.1074/jbc.M606057200
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