1ol0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1ol0" [edit=sysop:move=sysop])
Current revision (07:33, 23 October 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ol0.png|left|200px]]
 
-
<!--
+
==Crystal structure of a camelised human VH==
-
The line below this paragraph, containing "STRUCTURE_1ol0", creates the "Structure Box" on the page.
+
<StructureSection load='1ol0' size='340' side='right'caption='[[1ol0]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1ol0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OL0 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_1ol0| PDB=1ol0 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ol0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ol0 OCA], [https://pdbe.org/1ol0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ol0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ol0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ol0 ProSAT]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1ol0_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ol0 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The variable domain of dromedary immunoglobulins comprises only the heavy chain and is missing the light-chain variable domain. This single domain is sufficient for antigen recognition and binding-half that required by other mammals. Human antibody-VHs have previously been camelized to be soluble stable fragments that retain antigen binding. Such engineered VHH are of interest in drug development, since they are nonimmunogenic, and in other biotechnology applications. We present the structure of a camelized human antibody fragment (cVH), which is a competitive and reversible inhibitor of the NS3 serine protease of the hepatitis C virus (HCV). In solution, this cVH undergoes a concentration-dependent monomer-dimer equilibrium. The structure confirms the minimum mutational requirements of the VL-binding face. The fragment also suggests a means by which the observed dimerization occurs, highlighting the importance of the composition of the CDR3 in maintaining a truly camelized VH.
-
===CRYSTAL STRUCTURE OF A CAMELISED HUMAN VH===
+
Crystal structure of a human VH: requirements for maintaining a monomeric fragment.,Dottorini T, Vaughan CK, Walsh MA, LoSurdo P, Sollazzo M Biochemistry. 2004 Jan 27;43(3):622-8. PMID:14730966<ref>PMID:14730966</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_14730966}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1ol0" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 14730966 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_14730966}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
[[1ol0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OL0 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:14730966</ref><ref group="xtra">PMID:10702283</ref><ref group="xtra">PMID:9215580</ref><ref group="xtra">PMID:8683598</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Dottorini, T.]]
+
[[Category: Large Structures]]
-
[[Category: Losurdo, P.]]
+
[[Category: Dottorini T]]
-
[[Category: Sollazzo, M.]]
+
[[Category: Losurdo P]]
-
[[Category: Vaughan, C K.]]
+
[[Category: Sollazzo M]]
-
[[Category: Walsh, M A.]]
+
[[Category: Vaughan CK]]
-
[[Category: Camelised variable heavy domain]]
+
[[Category: Walsh MA]]
-
[[Category: Immunoglobulin]]
+

Current revision

Crystal structure of a camelised human VH

PDB ID 1ol0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ol0"
Personal tools