2p5t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2p5t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p5t, resolution 3.20&Aring;" /> '''Molecular and struct...)
Current revision (09:08, 21 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2p5t.gif|left|200px]]<br /><applet load="2p5t" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2p5t, resolution 3.20&Aring;" />
 
-
'''Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae'''<br />
 
-
==Overview==
+
==Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae==
-
The chromosomal pezT gene of the Gram-positive pathogen Streptococcus, pneumoniae encodes a protein that is homologous to the zeta toxin of the, Streptococcus pyogenes plasmid pSM19035-encoded epsilon-zeta, toxin-antitoxin system. Overexpression of pezT in Escherichia coli led to, severe growth inhibition from which the bacteria recovered approximately 3, h after induction of expression. The toxicity of PezT was counteracted by, PezA, which is encoded immediately upstream of pezT and shares weak, sequence similarities in the C-terminal region with the epsilon antitoxin., The pezAT genes form a bicistronic operon that is co-transcribed from a, sigma(70)-like promoter upstream of pezA and is negatively autoregulated, with PezA functioning as a transcriptional repressor and PezT as a, co-repressor. Both PezA and the non-toxic PezA(2)PezT(2) protein complex, bind to a palindrome sequence that overlaps the promoter. This differs, from the epsilon-zeta system in which epsilon functions solely as the, antitoxin and transcriptional regulation is carried out by another protein, designated omega. Results from site-directed mutagenesis experiments, demonstrated that the toxicity of PezT is dependent on a highly conserved, phosphoryltransferase active site and an ATP/GTP nucleotide binding site., In the PezA(2)PezT(2) complex, PezA neutralizes the toxicity of PezT by, blocking the nucleotide binding site through steric hindrance.
+
<StructureSection load='2p5t' size='340' side='right'caption='[[2p5t]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[2p5t]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] and [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P5T FirstGlance]. <br>
-
2P5T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P5T OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p5t OCA], [https://pdbe.org/2p5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p5t RCSB], [https://www.ebi.ac.uk/pdbsum/2p5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p5t ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae., Khoo SK, Loll B, Chan WT, Shoeman RL, Ngoo L, Yeo CC, Meinhart A, J Biol Chem. 2007 Jul 6;282(27):19606-18. Epub 2007 May 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17488720 17488720]
+
== Function ==
-
[[Category: Protein complex]]
+
[https://www.uniprot.org/uniprot/PEZA_STRPN PEZA_STRPN] Antitoxin component of a toxin-antitoxin (TA) module. Upon expression in E.coli neutralizes the toxic effect of cognate toxin PezT. Represses transcription of its own operon, PezT acts as a corepressor, considerably increasing repression.<ref>PMID:17488720</ref> <ref>PMID:21445328</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p5/2p5t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p5t ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
-
[[Category: Loll, B.]]
+
[[Category: Streptococcus pneumoniae TIGR4]]
-
[[Category: Meinhart, A.]]
+
[[Category: Loll B]]
-
[[Category: helix-turn-helix motif]]
+
[[Category: Meinhart A]]
-
[[Category: phosphoryltransferase]]
+
-
[[Category: postsegregational killing system]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:19:22 2008''
+

Current revision

Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae

PDB ID 2p5t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p5t"
Personal tools