1o7i

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[[Image:1o7i.png|left|200px]]
 
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==Crystal structure of a single stranded DNA binding protein==
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The line below this paragraph, containing "STRUCTURE_1o7i", creates the "Structure Box" on the page.
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<StructureSection load='1o7i' size='340' side='right'caption='[[1o7i]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1o7i| PDB=1o7i | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [https://pdbe.org/1o7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [https://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.
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===CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN===
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Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.,Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373<ref>PMID:12773373</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12773373}}, adds the Publication Abstract to the page
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12773373 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12773373}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA].
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: Kerr ID]]
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==Reference==
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[[Category: Naismith JH]]
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<ref group="xtra">PMID:12773373</ref><ref group="xtra">PMID:11526322</ref><references group="xtra"/>
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[[Category: Sulfolobus solfataricus]]
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[[Category: Kerr, I D.]]
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[[Category: Naismith, J H.]]
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[[Category: Dna-binding protein]]
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[[Category: Ob fold]]
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[[Category: Single stranded dna]]
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Current revision

Crystal structure of a single stranded DNA binding protein

PDB ID 1o7i

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