2uuz

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[[Image:2uuz.gif|left|200px]]<br /><applet load="2uuz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2uuz, resolution 2.3&Aring;" />
 
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'''ORTHORHOMBIC CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.'''<br />
 
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==Overview==
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==Orthorhombic crystal form of GamS from bacteriophage lambda.==
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In Escherichia coli, RecBCD processes double-stranded DNA breaks during, the initial stages of homologous recombination. RecBCD contains helicase, and nuclease activities, and unwinds and digests the blunt-ended DNA until, a specific eight-nucleotide sequence, Chi, is encountered. Chi modulates, the nuclease activity of RecBCD and results in a resected DNA end, which, is a substrate for RecA during subsequent steps in recombination. RecBCD, also acts as a defence mechanism against bacteriophage infection by, digesting linear viral DNA present during virus replication or resulting, from the action of restriction endonucleases. To avoid this fate, bacteriophage lambda encodes the gene Gam whose product is an inhibitor of, RecBCD. Gam has been shown to bind to RecBCD and inhibit its helicase and, nuclease activities. We show that Gam inhibits RecBCD by preventing it, from binding DNA. We have solved the crystal structure of Gam from two, different crystal forms. Using the published crystal structure of RecBCD, in complex with DNA we suggest models for the molecular mechanism of, Gam-mediated inhibition of RecBCD. We also propose that Gam could be a, mimetic of single-stranded, and perhaps also double-stranded, DNA.
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<StructureSection load='2uuz' size='340' side='right'caption='[[2uuz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2uuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uuz OCA], [https://pdbe.org/2uuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uuz RCSB], [https://www.ebi.ac.uk/pdbsum/2uuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uuz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAM_LAMBD GAM_LAMBD] Binds to host RecBCD nuclease and inhibits it thereby protecting the viral DNA against recBCD mediated degradation.<ref>PMID:17544443</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/2uuz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uuz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Escherichia coli, RecBCD processes double-stranded DNA breaks during the initial stages of homologous recombination. RecBCD contains helicase and nuclease activities, and unwinds and digests the blunt-ended DNA until a specific eight-nucleotide sequence, Chi, is encountered. Chi modulates the nuclease activity of RecBCD and results in a resected DNA end, which is a substrate for RecA during subsequent steps in recombination. RecBCD also acts as a defence mechanism against bacteriophage infection by digesting linear viral DNA present during virus replication or resulting from the action of restriction endonucleases. To avoid this fate, bacteriophage lambda encodes the gene Gam whose product is an inhibitor of RecBCD. Gam has been shown to bind to RecBCD and inhibit its helicase and nuclease activities. We show that Gam inhibits RecBCD by preventing it from binding DNA. We have solved the crystal structure of Gam from two different crystal forms. Using the published crystal structure of RecBCD in complex with DNA we suggest models for the molecular mechanism of Gam-mediated inhibition of RecBCD. We also propose that Gam could be a mimetic of single-stranded, and perhaps also double-stranded, DNA.
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==About this Structure==
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The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition.,Court R, Cook N, Saikrishnan K, Wigley D J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443<ref>PMID:17544443</ref>
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2UUZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUZ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The Crystal Structure of lambda-Gam Protein Suggests a Model for RecBCD Inhibition., Court R, Cook N, Saikrishnan K, Wigley D, J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17544443 17544443]
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</div>
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[[Category: Enterobacteria phage lambda]]
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<div class="pdbe-citations 2uuz" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Cook, N.]]
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<references/>
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[[Category: Court, R.I.]]
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__TOC__
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[[Category: Saikrishnan, K.]]
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</StructureSection>
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[[Category: Wigley, D.B.]]
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[[Category: Escherichia virus Lambda]]
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[[Category: bacteriophage lambda]]
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[[Category: Large Structures]]
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[[Category: inhibitor]]
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[[Category: Cook N]]
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[[Category: nuclease inhibitor]]
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[[Category: Court RI]]
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[[Category: putative dna mimic]]
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[[Category: Saikrishnan K]]
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[[Category: recbcd inhibitor]]
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[[Category: Wigley DB]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:23:36 2008''
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Current revision

Orthorhombic crystal form of GamS from bacteriophage lambda.

PDB ID 2uuz

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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