2q58

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2q58" [edit=sysop:move=sysop])
Current revision (11:20, 30 August 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2q58.png|left|200px]]
 
-
<!--
+
==Cryptosporidium parvum putative polyprenyl pyrophosphate synthase (cgd4_2550) in complex with zoledronate==
-
The line below this paragraph, containing "STRUCTURE_2q58", creates the "Structure Box" on the page.
+
<StructureSection load='2q58' size='340' side='right'caption='[[2q58]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2q58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum_Iowa_II Cryptosporidium parvum Iowa II]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2her 2her]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q58 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZOL:ZOLEDRONIC+ACID'>ZOL</scene></td></tr>
-
{{STRUCTURE_2q58| PDB=2q58 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q58 OCA], [https://pdbe.org/2q58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q58 RCSB], [https://www.ebi.ac.uk/pdbsum/2q58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q58 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q5CR09_CRYPI Q5CR09_CRYPI]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/2q58_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q58 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Cryptosporidiosis is a neglected disease without a wholly effective drug. We present a study demonstrating nitrogen-containing bisphosphonates (N-BPs) to be capable of inhibiting Cryptosporidium parvum at low micromolar concentrations in infected MDCK cells. Predictably, the mechanism of action is based on inhibition of biosynthesis of isoprenoids but the target enzyme is unexpectedly a distinctive C. parvum enzyme dubbed nonspecific polyprenyl pyrophosphate synthase (CpNPPPS). This enzyme produces various isoprenoid products larger than FPP and is inhibited by N-BPs at subnanomolar concentrations. It is part of an isoprenoid pathway in Cryptosporidium distinctly different from other organisms. The proposed mechanism of action is corroborated by crystal structures of the enzyme with risedronate and zoledronate bound showing how this enzyme's unique chain length determinant region enables it to accommodate larger substrates and products. These results, combined with existing data on their clinical use, demonstrate that N-BPs are very promising anticryptosporidial drug candidates.
-
===Cryptosporidium parvum putative polyprenyl pyrophosphate synthase (cgd4_2550) in complex with zoledronate===
+
Targeting a uniquely nonspecific prenyl synthase with bisphosphonates to combat cryptosporidiosis.,Artz JD, Dunford JE, Arrowood MJ, Dong A, Chruszcz M, Kavanagh KL, Minor W, Russell RG, Ebetino FH, Oppermann U, Hui R Chem Biol. 2008 Dec 22;15(12):1296-306. PMID:19101474<ref>PMID:19101474</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19101474}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2q58" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 19101474 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19101474}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Cryptosporidium parvum Iowa II]]
-
[[2q58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum_iowa_ii Cryptosporidium parvum iowa ii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2her 2her]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q58 OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Arrowsmith C]]
-
==Reference==
+
[[Category: Artz J]]
-
<ref group="xtra">PMID:19101474</ref><ref group="xtra">PMID:17125854</ref><references group="xtra"/>
+
[[Category: Bochkarev A]]
-
[[Category: Cryptosporidium parvum iowa ii]]
+
[[Category: Chruszcz M]]
-
[[Category: Arrowsmith, C.]]
+
[[Category: Dong A]]
-
[[Category: Artz, J.]]
+
[[Category: Dunford J]]
-
[[Category: Bochkarev, A.]]
+
[[Category: Edwards A]]
-
[[Category: Chruszcz, M.]]
+
[[Category: Hui R]]
-
[[Category: Dong, A.]]
+
[[Category: Kavanaugh KL]]
-
[[Category: Dunford, J.]]
+
[[Category: Kozieradski I]]
-
[[Category: Edwards, A.]]
+
[[Category: Lew J]]
-
[[Category: Hui, R.]]
+
[[Category: Minor W]]
-
[[Category: Kavanaugh, K L.]]
+
[[Category: Opperman U]]
-
[[Category: Kozieradski, I.]]
+
[[Category: Sundstrom M]]
-
[[Category: Lew, J.]]
+
[[Category: Weigelt J]]
-
[[Category: Minor, W.]]
+
[[Category: Zhao Y]]
-
[[Category: Opperman, U.]]
+
[[Category: Zheng H]]
-
[[Category: SGC, Structural Genomics Consortium.]]
+
-
[[Category: Sundstrom, M.]]
+
-
[[Category: Weigelt, J.]]
+
-
[[Category: Zhao, Y.]]
+
-
[[Category: Zheng, H.]]
+
-
[[Category: Farnesyl diphosphate synthase]]
+
-
[[Category: Sgc]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Structural genomics consortium]]
+
-
[[Category: Transferase]]
+

Current revision

Cryptosporidium parvum putative polyprenyl pyrophosphate synthase (cgd4_2550) in complex with zoledronate

PDB ID 2q58

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2q58"
Personal tools