3a7n

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[[Image:3a7n.png|left|200px]]
 
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==Crystal structure of uracil-DNA glycosylase from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_3a7n", creates the "Structure Box" on the page.
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<StructureSection load='3a7n' size='340' side='right'caption='[[3a7n]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3a7n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A7N FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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{{STRUCTURE_3a7n| PDB=3a7n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a7n OCA], [https://pdbe.org/3a7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a7n RCSB], [https://www.ebi.ac.uk/pdbsum/3a7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a7n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UNG_MYCTU UNG_MYCTU] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/3a7n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a7n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Uracil N-glycosylase (Ung) is the most thoroughly studied of the group of uracil DNA-glycosylase (UDG) enzymes that catalyse the first step in the uracil excision-repair pathway. The overall structure of the enzyme from Mycobacterium tuberculosis is essentially the same as that of the enzyme from other sources. However, differences exist in the N- and C-terminal stretches and some catalytic loops. Comparison with appropriate structures indicate that the two-domain enzyme closes slightly when binding to DNA, while it opens slightly when binding to the proteinaceous inhibitor Ugi. The structural changes in the catalytic loops on complexation reflect the special features of their structure in the mycobacterial protein. A comparative analysis of available sequences of the enzyme from different sources indicates high conservation of amino-acid residues in the catalytic loops. The uracil-binding pocket in the structure is occupied by a citrate ion. The interactions of the citrate ion with the protein mimic those of uracil, in addition to providing insights into other possible interactions that inhibitors could be involved in.
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===Crystal structure of uracil-DNA glycosylase from Mycobacterium tuberculosis===
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Structure of uracil-DNA glycosylase from Mycobacterium tuberculosis: insights into interactions with ligands.,Kaushal PS, Talawar RK, Varshney U, Vijayan M Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt, 8):887-92. Epub 2010 Jul 27. PMID:20693660<ref>PMID:20693660</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3a7n" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20693660}}, adds the Publication Abstract to the page
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20693660 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20693660}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3a7n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A7N OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Kaushal PS]]
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==Reference==
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[[Category: Talawar RK]]
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<ref group="xtra">PMID:20693660</ref><ref group="xtra">PMID:18453691</ref><ref group="xtra">PMID:12136137</ref><ref group="xtra">PMID:9776748</ref><references group="xtra"/>
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[[Category: Varshney U]]
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[[Category: Mycobacterium tuberculosis h37rv]]
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[[Category: Vijayan M]]
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[[Category: Uridine nucleosidase]]
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[[Category: Kaushal, P S.]]
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[[Category: Talawar, R K.]]
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[[Category: Varshney, U.]]
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[[Category: Vijayan, M.]]
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[[Category: Citrate as protein ligand]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Inhibitor design]]
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[[Category: Ligand binding]]
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[[Category: Ung-dna complex]]
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[[Category: Ung-ugi interaction]]
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Current revision

Crystal structure of uracil-DNA glycosylase from Mycobacterium tuberculosis

PDB ID 3a7n

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Proteopedia Page Contributors and Editors (what is this?)

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