3h3x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3h3x" [edit=sysop:move=sysop])
Current revision (07:59, 9 October 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3h3x.png|left|200px]]
 
-
<!--
+
==Structure of the V74M large subunit mutant of NI-FE hydrogenase in an oxidized state==
-
The line below this paragraph, containing "STRUCTURE_3h3x", creates the "Structure Box" on the page.
+
<StructureSection load='3h3x' size='340' side='right'caption='[[3h3x]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3h3x]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Solidesulfovibrio_fructosivorans Solidesulfovibrio fructosivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H3X FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FCO:CARBONMONOXIDE-(DICYANO)+IRON'>FCO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-
{{STRUCTURE_3h3x| PDB=3h3x | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h3x OCA], [https://pdbe.org/3h3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h3x RCSB], [https://www.ebi.ac.uk/pdbsum/3h3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h3x ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PHNS_SOLFR PHNS_SOLFR] Involved in hydrogen uptake for the anaerobic reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is the physiological electron acceptor.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h3/3h3x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h3x ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Hydrogenases catalyze the conversion between 2H(+) + 2e(-) and H(2)(1). Most of these enzymes are inhibited by O(2), which represents a major drawback for their use in biotechnological applications. (1, 2) Improving hydrogenase O(2) tolerance is therefore a major contemporary challenge to allow the implementation of a sustainable hydrogen economy. We succeeded in improving O(2) tolerance, which we define here as the ability of the enzyme to resist for several minutes to O(2) exposure, by substituting with methionines small hydrophobic residues strongly conserved in the gas channel. Remarkably, the mutated enzymes remained active in the presence of an O(2) concentration close to that found in aerobic solutions in equilibrium with air, while the wild type enzyme is inhibited in a few seconds. Crystallographic and spectroscopic studies showed that the structure and the chemistry at the active site are not affected by the mutations. Kinetic studies demonstrated that the inactivation is slower and reactivation faster in these mutants. We propose that in addition to restricting O(2) diffusion to the active site of the enzyme, methionine may also interact with bound peroxide and provide an assisted escape route for H(2)O(2) toward the gas channel. These results show for the first time that it is possible to improve O(2)-tolerance of [NiFe] hydrogenases, making possible the development of biohydrogen production systems.
-
===Structure of the V74M large subunit mutant of NI-FE hydrogenase in an oxidized state===
+
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.,Dementin S, Leroux F, Cournac L, Lacey AL, Volbeda A, Leger C, Burlat B, Martinez N, Champ S, Martin L, Sanganas O, Haumann M, Fernandez VM, Guigliarelli B, Fontecilla-Camps JC, Rousset M J Am Chem Soc. 2009 Jul 6. PMID:19580279<ref>PMID:19580279</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19580279}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3h3x" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 19580279 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19580279}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
[[3h3x]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_fructosovorans Desulfovibrio fructosovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H3X OCA].
+
[[Category: Solidesulfovibrio fructosivorans]]
-
 
+
[[Category: Fontecilla-Camps JC]]
-
==Reference==
+
[[Category: Martin L]]
-
<ref group="xtra">PMID:19580279</ref><ref group="xtra">PMID:9228943</ref><ref group="xtra">PMID:18685111</ref><references group="xtra"/>
+
[[Category: Martinez N]]
-
[[Category: Cytochrome-c3 hydrogenase]]
+
[[Category: Volbeda A]]
-
[[Category: Desulfovibrio fructosovorans]]
+
-
[[Category: Fontecilla-Camps, J C.]]
+
-
[[Category: Martin, L.]]
+
-
[[Category: Martinez, N.]]
+
-
[[Category: Volbeda, A.]]
+
-
[[Category: 3fe-4]]
+
-
[[Category: 4fe-4]]
+
-
[[Category: Iron]]
+
-
[[Category: Iron-sulfur]]
+
-
[[Category: Metal-binding]]
+
-
[[Category: Ni-fe hydrogenase tunnel mutant]]
+
-
[[Category: Nickel]]
+
-
[[Category: Oxidoreductase]]
+
-
[[Category: Periplasm]]
+

Current revision

Structure of the V74M large subunit mutant of NI-FE hydrogenase in an oxidized state

PDB ID 3h3x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools