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1h9i

From Proteopedia

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[[Image:1h9i.png|left|200px]]
 
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==COMPLEX OF EETI-II MUTANT WITH PORCINE TRYPSIN==
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The line below this paragraph, containing "STRUCTURE_1h9i", creates the "Structure Box" on the page.
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<StructureSection load='1h9i' size='340' side='right'caption='[[1h9i]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1h9i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H9I FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_1h9i| PDB=1h9i | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9i OCA], [https://pdbe.org/1h9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h9i RCSB], [https://www.ebi.ac.uk/pdbsum/1h9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.
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===COMPLEX OF EETI-II MUTANT WITH PORCINE TRYPSIN===
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Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.,Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759<ref>PMID:16131759</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1h9i" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16131759}}, adds the Publication Abstract to the page
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16131759 is the PubMed ID number.
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*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_16131759}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[1h9i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9I OCA].
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[[Category: Ecballium elaterium]]
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:16131759</ref><ref group="xtra">PMID:10409654</ref><ref group="xtra">PMID:10441159</ref><ref group="xtra">PMID:2622910</ref><references group="xtra"/>
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Trypsin]]
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[[Category: Kolmar H]]
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[[Category: Kolmar, H.]]
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[[Category: Kraetzner R]]
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[[Category: Kraetzner, R.]]
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[[Category: Uson I]]
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[[Category: Uson, I.]]
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[[Category: Wentzel A]]
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[[Category: Wentzel, A.]]
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[[Category: Cystine knot]]
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[[Category: Hydrolase/inhibitor]]
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[[Category: Squash inhibitor]]
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[[Category: Trypsin]]
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Current revision

COMPLEX OF EETI-II MUTANT WITH PORCINE TRYPSIN

PDB ID 1h9i

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