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2c5d

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[[Image:2c5d.png|left|200px]]
 
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==Structure of a minimal Gas6-Axl complex==
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The line below this paragraph, containing "STRUCTURE_2c5d", creates the "Structure Box" on the page.
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<StructureSection load='2c5d' size='340' side='right'caption='[[2c5d]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c5d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C5D FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2c5d| PDB=2c5d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c5d OCA], [https://pdbe.org/2c5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c5d RCSB], [https://www.ebi.ac.uk/pdbsum/2c5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c5d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAS6_HUMAN GAS6_HUMAN] Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.<ref>PMID:12364394</ref> <ref>PMID:18840707</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c5/2c5d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c5d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Receptor tyrosine kinases of the Axl family are activated by the vitamin K-dependent protein Gas6. Axl signalling plays important roles in cancer, spermatogenesis, immunity, and platelet function. The crystal structure at 3.3 A resolution of a minimal human Gas6/Axl complex reveals an assembly of 2:2 stoichiometry, in which the two immunoglobulin-like domains of the Axl ectodomain are crosslinked by the first laminin G-like domain of Gas6, with no direct Axl/Axl or Gas6/Gas6 contacts. There are two distinct Gas6/Axl contacts of very different size, both featuring interactions between edge beta-strands. Structure-based mutagenesis, protein binding assays and receptor activation experiments demonstrate that both the major and minor Gas6 binding sites are required for productive transmembrane signalling. Gas6-mediated Axl dimerisation is likely to occur in two steps, with a high-affinity 1:1 Gas6/Axl complex forming first. Only the minor Gas6 binding site is highly conserved in the other Axl family receptors, Sky/Tyro3 and Mer. Specificity at the major contact is suggested to result from the segregation of charged and apolar residues to opposite faces of the newly formed beta-sheet.
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===STRUCTURE OF A MINIMAL GAS6-AXL COMPLEX===
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Structural basis for Gas6-Axl signalling.,Sasaki T, Knyazev PG, Clout NJ, Cheburkin Y, Gohring W, Ullrich A, Timpl R, Hohenester E EMBO J. 2006 Jan 11;25(1):80-7. Epub 2005 Dec 15. PMID:16362042<ref>PMID:16362042</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16362042}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2c5d" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16362042 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16362042}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2c5d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5D OCA].
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==Reference==
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<ref group="xtra">PMID:16362042</ref><ref group="xtra">PMID:12218057</ref><ref group="xtra">PMID:14623883</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Transferase]]
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[[Category: Large Structures]]
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[[Category: Cheburkin, Y.]]
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[[Category: Cheburkin Y]]
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[[Category: Clout, N J.]]
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[[Category: Clout NJ]]
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[[Category: Goehring, W.]]
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[[Category: Goehring W]]
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[[Category: Hohenester, E.]]
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[[Category: Hohenester E]]
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[[Category: Knyazev, P G.]]
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[[Category: Knyazev PG]]
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[[Category: Sasaki, T.]]
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[[Category: Sasaki T]]
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[[Category: Timpl, R.]]
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[[Category: Timpl R]]
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[[Category: Ullrich, A.]]
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[[Category: Ullrich A]]
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[[Category: Egf-like domain]]
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[[Category: Growth regulation]]
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[[Category: Growth regulation/complex]]
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[[Category: Immunoglobulin-like domain]]
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[[Category: Laminin g-like domain]]
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[[Category: Receptor]]
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[[Category: Receptor tyrosine kinase]]
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[[Category: Signaling protein/receptor]]
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[[Category: Vitamin k-dependent protein]]
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Current revision

Structure of a minimal Gas6-Axl complex

PDB ID 2c5d

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