3l9g

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[[Image:3l9g.png|left|200px]]
 
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==Urate oxidase complexed with uric acid and chloride==
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The line below this paragraph, containing "STRUCTURE_3l9g", creates the "Structure Box" on the page.
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<StructureSection load='3l9g' size='340' side='right'caption='[[3l9g]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3l9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L9G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=URC:URIC+ACID'>URC</scene></td></tr>
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{{STRUCTURE_3l9g| PDB=3l9g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l9g OCA], [https://pdbe.org/3l9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l9g RCSB], [https://www.ebi.ac.uk/pdbsum/3l9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l9g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l9/3l9g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l9g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Urate oxidase (uricase; EC 1.7.3.3; UOX) from Aspergillus flavus catalyzes the oxidation of uric acid in the presence of molecular oxygen to 5-hydroxyisourate in the degradation cascade of purines; intriguingly, catalysis proceeds using neither a metal ion (Fe, Cu etc.) nor a redox cofactor. UOX is a tetrameric enzyme with four active sites located at the interface of two subunits; its structure was refined at atomic resolution (1 A) using new crystal data in the presence of xanthine and at near-atomic resolution (1.3-1.7 A) in complexes with the natural substrate (urate) and two inhibitors: 8-nitroxanthine and 8-thiouric acid. Three new features of the structural and mechanistic behaviour of the enzyme were addressed. Firstly, the high resolution of the UOX-xanthine structure allowed the solution of an old structural problem at a contact zone within the tetramer; secondly, the protonation state of the substrate was determined from both a halochromic inhibitor complex (UOX-8-nitroxanthine) and from the H-atom distribution in the active site, using the structures of the UOX-xanthine and the UOX-uric acid complexes; and thirdly, it was possible to extend the general base system, characterized by the conserved catalytic triad Thr-Lys-His, to a large water network that is able to buffer and shuttle protons back and forth between the substrate and the peroxo hole along the reaction pathway.
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===Urate oxidase complexed with uric acid and chloride===
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Near-atomic resolution structures of urate oxidase complexed with its substrate and analogues: the protonation state of the ligand.,Gabison L, Chiadmi M, El Hajji M, Castro B, Colloc'h N, Prange T Acta Crystallogr D Biol Crystallogr. 2010 Jun;66(Pt 6):714-24. Epub 2010 May 15. PMID:20516624<ref>PMID:20516624</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3l9g" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16545381}}, adds the Publication Abstract to the page
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*[[Urate oxidase 3D structures|Urate oxidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16545381 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16545381}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3l9g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L9G OCA].
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==Reference==
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<ref group="xtra">PMID:16545381</ref><ref group="xtra">PMID:18638417</ref><ref group="xtra">PMID:18375516</ref><references group="xtra"/>
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[[Category: Aspergillus flavus]]
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[[Category: Urate oxidase]]
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[[Category: Gabison, L.]]
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[[Category: Prange, T.]]
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[[Category: H, N Colloc.]]
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[[Category: Acetylation]]
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[[Category: Aspergillus flavus]]
[[Category: Aspergillus flavus]]
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[[Category: High resolution]]
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[[Category: Large Structures]]
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[[Category: Oxidoreductase]]
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[[Category: Colloc'h N]]
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[[Category: Peroxisome]]
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[[Category: Gabison L]]
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[[Category: Purine metabolism]]
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[[Category: Prange T]]
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[[Category: Urate oxidase]]
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[[Category: Uric acid]]
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Current revision

Urate oxidase complexed with uric acid and chloride

PDB ID 3l9g

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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