2eb5

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(New page: 200px<br /><applet load="2eb5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2eb5, resolution 1.70&Aring;" /> '''Crystal structure of...)
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[[Image:2eb5.jpg|left|200px]]<br /><applet load="2eb5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2eb5, resolution 1.70&Aring;" />
 
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'''Crystal structure of HpcG complexed with oxalate'''<br />
 
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==Overview==
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==Crystal structure of HpcG complexed with oxalate==
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HpcG catalyses the hydration of a carbon-carbon double bond without the, aid of any cofactor other than a simple divalent metal ion such as Mg(2+)., Since the substrate has a nearby carbonyl group, it is believed that it, first isomerises to form a pair of conjugated double bonds in the enol, tautomer before Michael addition of water. Previous chemical studies of, the reaction, and that of the related enzyme MhpD, have failed to provide, a clear picture of the mechanism. The substrate itself is unstable, preventing co-crystallisation or soaking of crystals, but oxalate is a, strong competitive inhibitor. We have solved the crystal structure of the, protein in the apo form, and with magnesium and oxalate bound. Modelling, substrate into the active site suggests the attacking water molecule is, not part of the metal coordination shell, in contrast to a previous, proposal. Our model suggests that geometrically strained cis isomer, intermediates do not lie on the reaction pathway, and that separate groups, are involved in the isomerisation and hydration steps.
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<StructureSection load='2eb5' size='340' side='right'caption='[[2eb5]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2eb5]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EB5 FirstGlance]. <br>
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2EB5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=OXL:'>OXL</scene> and <scene name='pdbligand=SCN:'>SCN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EB5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eb5 OCA], [https://pdbe.org/2eb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eb5 RCSB], [https://www.ebi.ac.uk/pdbsum/2eb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eb5 ProSAT]</span></td></tr>
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Structure and Mechanism of HpcG, a Hydratase in the Homoprotocatechuate Degradation Pathway of Escherichia coli., Izumi A, Rea D, Adachi T, Unzai S, Park SY, Roper DI, Tame JR, J Mol Biol. 2007 Jul 27;370(5):899-911. Epub 2007 May 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17559873 17559873]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q46982_ECOLX Q46982_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2eb5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eb5 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Adachi, T.]]
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[[Category: Adachi T]]
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[[Category: Izumi, A.]]
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[[Category: Izumi A]]
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[[Category: Park, S.Y.]]
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[[Category: Park SY]]
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[[Category: Rea, D.]]
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[[Category: Rea D]]
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[[Category: Roper, D.I.]]
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[[Category: Roper DI]]
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[[Category: Tame, J.R.H.]]
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[[Category: Tame JRH]]
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[[Category: Unzai, S.]]
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[[Category: Unzai S]]
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[[Category: MG]]
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[[Category: OXL]]
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[[Category: SCN]]
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[[Category: hydratase]]
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[[Category: lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:30:25 2008''
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Current revision

Crystal structure of HpcG complexed with oxalate

PDB ID 2eb5

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