3eqo

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[[Image:3eqo.png|left|200px]]
 
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==Crystal structure of beta-1,3-glucanase from Phanerochaete chrysosporium (Lam55A) gluconolactone complex==
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The line below this paragraph, containing "STRUCTURE_3eqo", creates the "Structure Box" on the page.
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<StructureSection load='3eqo' size='340' side='right'caption='[[3eqo]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3eqo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EQO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=LGC:(3S,4R,5R,6S)-3,4,5-TRIHYDROXY-6-(HYDROXYMETHYL)TETRAHYDRO-2H-PYRAN-2-ONE'>LGC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3eqo| PDB=3eqo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eqo OCA], [https://pdbe.org/3eqo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eqo RCSB], [https://www.ebi.ac.uk/pdbsum/3eqo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eqo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q2Z1W1_PHACH Q2Z1W1_PHACH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/3eqo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eqo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycoside hydrolase family 55 consists of beta-1,3-glucanases mainly from filamentous fungi. A beta-1,3-glucanase (Lam55A) from the Basidiomycete Phanerochaete chrysosporium hydrolyzes beta-1,3-glucans in the exo-mode with inversion of anomeric configuration and produces gentiobiose in addition to glucose from beta-1,3/1,6-glucans. Here we report the crystal structure of Lam55A, establishing the three-dimensional structure of a member of glycoside hydrolase 55 for the first time. Lam55A has two beta-helical domains in a single polypeptide chain. These two domains are separated by a long linker region but are positioned side by side, and the overall structure resembles a rib cage. In the complex, a gluconolactone molecule is bound at the bottom of a pocket between the two beta-helical domains. Based on the position of the gluconolactone molecule, Glu-633 appears to be the catalytic acid, whereas the catalytic base residue could not be identified. The substrate binding pocket appears to be able to accept a gentiobiose unit near the cleavage site, and a long cleft runs from the pocket, in accordance with the activity of this enzyme toward various beta-1,3-glucan oligosaccharides. In conclusion, we provide important features of the substrate-binding site at the interface of the two beta-helical domains, demonstrating an unexpected variety of carbohydrate binding modes.
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===Crystal structure of beta-1,3-glucanase from Phanerochaete chrysosporium (Lam55A) gluconolactone complex===
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Crystal structure of glycoside hydrolase family 55 {beta}-1,3-glucanase from the basidiomycete Phanerochaete chrysosporium.,Ishida T, Fushinobu S, Kawai R, Kitaoka M, Igarashi K, Samejima M J Biol Chem. 2009 Apr 10;284(15):10100-9. Epub 2009 Feb 4. PMID:19193645<ref>PMID:19193645</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3eqo" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19193645}}, adds the Publication Abstract to the page
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*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19193645 is the PubMed ID number.
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_19193645}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[3eqo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQO OCA].
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[[Category: Large Structures]]
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[[Category: Phanerodontia chrysosporium]]
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==Reference==
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[[Category: Fushinobu S]]
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<ref group="xtra">PMID:19193645</ref><ref group="xtra">PMID:16614901</ref><references group="xtra"/>
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[[Category: Igarashi K]]
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[[Category: Glucan 1,3-beta-glucosidase]]
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[[Category: Ishida T]]
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[[Category: Phanerochaete chrysosporium]]
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[[Category: Kawai R]]
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[[Category: Fushinobu, S.]]
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[[Category: Kitaoka M]]
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[[Category: Igarashi, K.]]
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[[Category: Samejima M]]
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[[Category: Ishida, T.]]
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[[Category: Kawai, R.]]
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[[Category: Kitaoka, M.]]
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[[Category: Samejima, M.]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Tandem beta-helix domain]]
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Current revision

Crystal structure of beta-1,3-glucanase from Phanerochaete chrysosporium (Lam55A) gluconolactone complex

PDB ID 3eqo

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