2jc2

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[[Image:2jc2.png|left|200px]]
 
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==The crystal structure of the natural F112L human sorcin mutant==
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The line below this paragraph, containing "STRUCTURE_2jc2", creates the "Structure Box" on the page.
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<StructureSection load='2jc2' size='340' side='right'caption='[[2jc2]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jc2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JC2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2jc2| PDB=2jc2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jc2 OCA], [https://pdbe.org/2jc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2jc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jc2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SORCN_HUMAN SORCN_HUMAN] Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.<ref>PMID:17699613</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/2jc2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jc2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The penta-EF hand protein sorcin participates in the modulation of Ca2+-induced calcium-release in the heart through the interaction with several Ca2+ channels such as the ryanodine receptor. The modulating activity is impaired in the recently described natural F112L mutant. The F112 residue is located at the end of the D helix next to Asp113, one of the calcium ligands in the EF3 hand endowed with the highest affinity for the metal. The F112L-sorcin X-ray crystal structure at 2.5 A resolution displays marked alterations in the EF3 hand, where the hydrogen bonding network established by Phe112 is disrupted, and in the EF1 region, which is tilted in both monomers that give rise to the dimer, the stable form of the molecule. In turn, the observed tilt is indicative of an increased flexibility of the N-terminal part of the molecule. The structural alterations result in a 6-fold decrease in calcium affinity with respect to the wild-type protein and to an even larger impairment of the interaction with annexin VII and of the ability of sorcin to interact with and inhibit ryanodine receptors. These results provide a plausible structural and functional framework that helps elucidate the phenotypic alterations of mice overexpressing F112L-sorcin.
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===THE CRYSTAL STRUCTURE OF THE NATURAL F112L HUMAN SORCIN MUTANT===
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Molecular basis for the impaired function of the natural F112L sorcin mutant: X-ray crystal structure, calcium affinity, and interaction with annexin VII and the ryanodine receptor.,Franceschini S, Ilari A, Verzili D, Zamparelli C, Antaramian A, Rueda A, Valdivia HH, Chiancone E, Colotti G FASEB J. 2008 Jan;22(1):295-306. Epub 2007 Aug 15. PMID:17699613<ref>PMID:17699613</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17699613}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2jc2" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17699613 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17699613}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2jc2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JC2 OCA].
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==Reference==
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<ref group="xtra">PMID:17699613</ref><ref group="xtra">PMID:11922676</ref><ref group="xtra">PMID:11714909</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Chiancone, E.]]
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[[Category: Large Structures]]
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[[Category: Colotti, G.]]
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[[Category: Chiancone E]]
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[[Category: Franceschini, S.]]
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[[Category: Colotti G]]
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[[Category: Ilari, A.]]
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[[Category: Franceschini S]]
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[[Category: Calcium]]
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[[Category: Ilari A]]
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[[Category: Calcium binding protein]]
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[[Category: Metal binding protein]]
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[[Category: Natural f112l sorcin mutant]]
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[[Category: Ryanodine receptor interacting protein]]
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Current revision

The crystal structure of the natural F112L human sorcin mutant

PDB ID 2jc2

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OCA

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