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2o9l
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="2o9l" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o9l" /> '''AMBER refined NMR Structure of the Sigma-54 ...) |
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| - | [[Image:2o9l.jpg|left|200px]]<br /><applet load="2o9l" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2o9l" /> | ||
| - | '''AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element'''<br /> | ||
| - | == | + | ==AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element== |
| - | The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene | + | <StructureSection load='2o9l' size='340' side='right'caption='[[2o9l]]' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2o9l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O9L FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o9l OCA], [https://pdbe.org/2o9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o9l RCSB], [https://www.ebi.ac.uk/pdbsum/2o9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o9l ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O66858_AQUAE O66858_AQUAE] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/2o9l_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o9l ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP. | ||
| - | + | Structural basis of DNA recognition by the alternative sigma-factor, sigma54.,Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658<ref>PMID:17481658</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2o9l" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Sigma factor 3D structures|Sigma factor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aquifex aeolicus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Doucleff M]] | ||
| + | [[Category: Lee PS]] | ||
| + | [[Category: Pelton JG]] | ||
| + | [[Category: Wemmer DE]] | ||
Current revision
AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
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